Spatio‐temporal expression of activating transcription factor 5 in the skeletal development of mouse limb
Spatio‐temporal expression of activating transcription factor 5 in the skeletal development of mouse limb
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DOI：
10.1111/j.1440-169x.2009.01126.x
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发表时间：
2009-08
期刊：
影响因子：
4.6
通讯作者：
H. Satake;Kazuo Ito;M. Takahara;Takashi Furukawa;M. Takagi;T. Ogino;T. Shinomura
中科院分区：
文献类型：
--
作者：
H. Satake;Kazuo Ito;M. Takahara;Takashi Furukawa;M. Takagi;T. Ogino;T. Shinomura
在肢体发育过程中，关于激活转录因子5（Atf5）的表达模式尚无相关数据。本研究的目的是描述Atf5在小鼠肢体发育过程中的表达情况。通过原位杂交检测了一些样本中Ⅱ型胶原蛋白α1（Col2a1）、性别决定区Y相关高迁移率族盒9（Sox9）和激活转录因子2（Atf2）的表达。通过实时定量聚合酶链反应检测了ATDC5细胞、小鼠肢芽和骺软骨中Atf5α和Atf5β的表达。在浓缩的间充质细胞、软骨原基、增殖性、肥大前和关节软骨细胞中检测到Atf5转录本，但在肥大软骨细胞中未检测到。Atf5主要由初级松质骨中的成骨细胞表达。在出生后阶段，Atf5在骺软骨细胞和骨小梁衬里的成骨细胞中表达。在发育中的肢体中，除骨组织外，Col2a1、Sox9和Atf2转录本的空间分布与Atf5的空间分布密切对应。在软骨中，Atf5α在胚胎期和出生后阶段都是主要形式。
There are no data about the expression pattern of activating transcription factor 5 (Atf5) during limb development. The aim of the present study was to describe the expression of Atf5 throughout mouse limb development. Some specimens were examined for the expression of type II collagen α1 (Col2a1), sex‐determining region Y‐related high‐mobility‐group‐box 9 (Sox9), and activating transcription factor 2 (Atf2) by in situ hybridization. ATDC5 cells, mouse limb buds, and epiphyseal cartilage were examined for the expression of Atf5α and Atf5β by quantitative real‐time PCR. Atf5 transcripts were detected in condensed mesenchymal cells, cartilage primordia, proliferative, prehypertrophic, and articular chondrocytes, but not in hypertrophic chondrocytes. Atf5 was predominantly expressed by osteoblasts in the primary spongiosa. At the postnatal stage, Atf5 was expressed in epiphyseal chondrocytes and osteoblasts lining the bone trabeculae. Spatial distribution of Col2a1, Sox9, and Atf2 transcripts closely corresponded to that of Atf5 within developing limbs, except for bony tissues. In cartilage, Atf5α is the dominant form in the embryonic stage as well as in the postnatal stage.