POLIOVIRUS TYPE-1 TYPE-3 ANTIGENIC HYBRID VIRUS CONSTRUCTED INVITRO ELICITS TYPE-1 AND TYPE-3 NEUTRALIZING ANTIBODIES IN RABBITS AND MONKEYS
POLIOVIRUS TYPE-1 TYPE-3 ANTIGENIC HYBRID VIRUS CONSTRUCTED INVITRO ELICITS TYPE-1 AND TYPE-3 NEUTRALIZING ANTIBODIES IN RABBITS AND MONKEYS
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DOI：
10.1073/pnas.85.9.3203
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
WIMMER, E
中科院分区：
文献类型：
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作者：
MURRAY, MG;KUHN, RJ;WIMMER, E
脊髓灰质炎病毒存在三种稳定的血清型（PV-1、PV-2和PV-3）。这些病毒显示三个抗原位点，分别命名为N-AgI、N-AgII和N-AgIII。当用脊髓灰质炎病毒免疫小鼠时，N-AgI是PV-3的主要中和抗原位点，而N-AgII和N-AgIII对PV-1的免疫显性超过N-AgI。为了研究结构与抗原性之间的关系，构建了一种杂交病毒，其中PV-1的N-AgI被PV-3的N-AgI取代。PV-3和PV-1特异性抗血清，包括在灵长类动物中由PV-3引起的抗血清，中和了杂交病毒。将杂交病毒注射到兔或灵长类动物中，产生中和PV-1和PV-3的抗尿素酶。数据显示脊髓灰质炎病毒N-AgI的序列替换与病毒增殖相容，这一观察结果可用于开发多价小核糖核酸病毒疫苗。
Poliovirus exists as three stable serotypes (PV-1, PV-2, and PV-3). These viruses display three antigenic sites each, designated N-AgI, N-AgII, and N-AgIII. When mice are immunized with poliovirus, N-AgI is the major neutralization antigenic site for PV-3, whereas N-AgII and N-AgIII are immunodominant over N-AgI for PV-1. To study the relationship between structure and antigenicity, a hybrid virus was constructed in which N-AgI of PV-1 was replaced by N-AgI of PV-3. PV-3- and PV-1-specific antisera, including those elicited by PV-3 in primates, neutralized the hybrid virus. Injection of the hybrid virus into rabbits or into primates resulted in the production of antisrea that neutralized both PV-1 and PV-3. The data show that sequence replacement at N-AgI of poliovirus is compatible with viral proliferation, an observation useful for the development of multivalent picornavirus vaccines.