Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation.
Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation.
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DOI：
10.1007/978-1-4939-3631-1_19
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发表时间：
2016
影响因子：
--
通讯作者：
Xindan Wang;Paula Montero Llopis
中科院分区：
文献类型：
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作者：
Xindan Wang;Paula Montero Llopis
枯草芽孢杆菌是研究细胞过程最常用的革兰氏阳性菌，因为它具有遗传上的易处理性。此外，在营养限制期间，B.枯草杆菌经历孢子形成的发育过程，这是细胞分化的最简单的例子之一。这些过程的许多方面都得益于荧光显微镜。在这里，我们描述了基本的宽视场荧光显微镜技术可视化B。营养生长过程中的枯草杆菌和孢子形成的发育过程。
Bacillus subtilisis the most commonly used Gram-positive bacterium to study cellular processes because of its genetic tractability. In addition, during nutrient limitation,B. subtilisundergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualizeB. subtilisduring vegetative growth, and the developmental process of sporulation.