Lipidomics reveals membrane lipid remodelling and release of potential lipid mediators during early stress responses in a murine melanoma cell line
Lipidomics reveals membrane lipid remodelling and release of potential lipid mediators during early stress responses in a murine melanoma cell line
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DOI：
10.1016/j.bbalip.2010.04.011
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Vigh, Laszlo
中科院分区：
文献类型：
--
作者：
Balogh, Gabor;Peter, Maria;Vigh, Laszlo
众所周知，膜可以通过改变其组成和微域组织来快速响应各种环境扰动。在之前的工作中，我们表明膜流化剂苯甲醇（BA）可以模拟热应激的影响并增强不同哺乳动物细胞中热休克蛋白的合成。在这里，我们通过表征小鼠黑色素瘤 1316 细胞中应激诱导的膜脂变化，进一步探讨热和 BA 诱导的应激。脂质组学指纹显示，通过热或 BA 实现的膜应激会导致脂质代谢发生明显且高度特异性的改变。多烯的损失以及饱和脂质种类的伴随增加被证明是磷脂酶（主要是磷脂酶 A(2) 和 C）激活的结果。在 B16 细胞中发现了磷脂酶 C-二酰基甘油脂肪酶-单酰基甘油脂肪酶途径，该途径对应激时多种脂质介质的产生有显着贡献，包括有效的热休克调节剂花生四烯酸。在 B16 细胞的热和 BA 处理中观察到胆固醇、神经酰胺和饱和磷酸甘油酯物质的积累，这些物质具有筏形成特性，这可能解释了先前通过荧光显微镜检测到的有序质膜域的浓缩，并且可能作为应激反应中的信号平台或作为针对应激有害影响的主要防御机制。 (C) 2010 Elsevier B.V. 保留所有权利。
Membranes are known to respond rapidly to various environmental perturbations by changing their composition and microdomain organization. In previous work we showed that a membrane fluidizer benzyl alcohol (BA) could mimic the effects of heat stress and enhance heat shock protein synthesis in different mammalian cells. Here we explore heat- and BA-induced stress further by characterizing stress-induced membrane lipid changes in mouse melanoma 1316 cells. Lipidomic fingerprints revealed that membrane stress achieved either by heat or BA resulted in pronounced and highly specific alterations in lipid metabolism. The loss in polyenes with the concomitant increase in saturated lipid species was shown to be a consequence of the activation of phopholipases (mainly phopholipase A(2) and C). A phospholipase C-diacylglycerol lipase-monoacylglycerol lipase pathway was identified in B16 cells and contributed significantly to the production of several lipid mediators upon stress including the potent heat shock modulator, arachidonic acid. The accumulation of cholesterol, ceramide and saturated phosphoglyceride species with raft-forming properties observed upon both heat and BA treatments of B16 cells may explain the condensation of ordered plasma membrane domains previously detected by fluorescence microscopy and may serve as a signalling platform in stress responses or as a primary defence mechanism against the noxious effects of stresses. (C) 2010 Elsevier B.V. All rights reserved.