Time-Transient Effects of Silver and Copper in the Porous Titanium Dioxide Layer on Antibacterial Properties
Time-Transient Effects of Silver and Copper in the Porous Titanium Dioxide Layer on Antibacterial Properties
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DOI：
10.3390/jfb11020044
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发表时间：
2020-06-01
影响因子：
4.8
通讯作者：
Hanawa, Takao
中科院分区：
文献类型：
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作者：
Shimabukuro, Masaya;Hiji, Akari;Hanawa, Takao
最近，银（Ag）和铜（Cu）已被掺入钛（Ti）表面以实现其抗菌性能。本研究研究了掺银和掺铜的多孔二氧化钛 (TiO2) 层的抗菌效果的持久性和表面变化。使用含有银和铜离子的电解质，通过微弧氧化 (MAO) 处理形成掺有银和铜的 TiO2 层。掺入银和铜的样本在盐水中孵育 0-28 天。使用 X 射线光电子能谱 (XPS) 表征 Ag 和 Cu 的浓度和化学状态的变化。对大肠杆菌（E. coli）抗菌效果的持久性采用国际标准化组织（ISO）方法进行评价。结果，通过MAO在Ti表面上形成掺入Ag和Cu的多孔TiO 2 层。 Ag 的化学状态从 Ag2O 变为金属 Ag，而 Cu 的化学状态在盐水中孵育长达 28 天没有变化。在盐水中孵育 28 天期间，Cu 以稳定的 Cu2O 化合物形式存在于 TiO2 层中。通过长达 7 天的孵育，Ag 和 Cu 的浓度急剧下降，并且在 28 天之前仍保持少量。掺有银的样本的抗菌作用减弱，而含铜的样本即使在盐水中孵育后仍保持抗菌作用。我们的研究表明银和铜的瞬态效应对于发挥其抗菌作用非常重要。
Recently, silver (Ag) and copper (Cu) have been incorporated into a titanium (Ti) surface to realize their antibacterial property. This study investigated both the durability of the antibacterial effect and the surface change of the Ag- and Cu-incorporated porous titanium dioxide (TiO2) layer. Ag- and Cu-incorporated TiO2 layers were formed by micro-arc oxidation (MAO) treatment using the electrolyte with Ag and Cu ions. Ag- and Cu-incorporated specimens were incubated in saline during a period of 0-28 days. The changes in both the concentrations and chemical states of the Ag and Cu were characterized using X-ray photoelectron spectroscopy (XPS). The durability of the antibacterial effects againstEscherichia coli(E. coli)were evaluated by the international organization for standardization (ISO) method. As a result, the Ag- and Cu-incorporated porous TiO2 layers were formed on a Ti surface by MAO. The chemical state of Ag changed from Ag2O to metallic Ag, whilst that of Cu did not change by incubation in saline for up to 28 days. Cu existed as a stable Cu2O compound in the TiO2 layer during the 28 days of incubation in saline. The concentrations of Ag and Cu were dramatically decreased by incubation for up to 7 days, and remained a slight amount until 28 days. The antibacterial effect of Ag-incorporated specimens diminished, and that of Cu was maintained even after incubation in saline. Our study suggests the importance of the time-transient effects of Ag and Cu on develop their antibacterial effects.