REL2, A Gene Encoding An Unknown Function Protein which Contains DUF630 and DUF632 Domains Controls Leaf Rolling in Rice.
REL2, A Gene Encoding An Unknown Function Protein which Contains DUF630 and DUF632 Domains Controls Leaf Rolling in Rice.
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REL2，一种编码未知功能蛋白的基因，包含控制水稻卷叶的 DUF630 和 DUF632 结构域
DOI：
10.1186/s12284-016-0105-6
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Chen KM
中科院分区：
文献类型：
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作者：
Yang SQ;Li WQ;Miao H;Gan PF;Qiao L;Chang YL;Shi CH;Chen KM
水稻叶片是水稻植株重要的能量来源。优化的叶片结构有利于水稻叶片吸收光能和进行气体交换，从而提高水稻产量。适度的卷叶和相对直立的植株结构可能有助于水稻的高产，但相关的分子机制仍然不清楚。ResultsIn这项研究中，我们确定并表征了一个水稻卷叶和直立叶突变体，并将其命名为asrel 2。组织学分析表明，突变体there 12的泡状细胞数量增加，中泡状细胞体积缩小。我们首先通过图位克隆的方法将p53 2定位于第10号染色体上一个35-kb的物理区域。进一步分析表明，该基因编码一个含有DUF 630和DUF 632结构域的蛋白。inrel 2突变体中DUF 630结构域的两个核苷酸替换突变导致了p53 2基因座的功能丧失，p53 2基因在inrel 2突变体中的互补表达证实了p53 2的功能。进一步的研究表明，水稻HSP 2蛋白主要分布在细胞质膜上，沿着分布，HSP 2基因在水稻幼叶中表达量相对较高。定量RT-PCR分析结果表明，该基因可能与泡状细胞发育、生长素合成和运输等相关基因存在功能连锁。这是质膜定位，其在控制叶片形态中的功能可能涉及到多个生物学过程，如泡状细胞发育和生长素的合成和运输。
BackgroundRice leaves are important energy source for the whole plant. An optimal structure will be beneficial for rice leaves to capture light energy and exchange gas, thus increasing the yield of rice. Moderate leaf rolling and relatively erect plant architecture may contribute to high yield of rice, but the relevant molecular mechanism remains unclear.ResultsIn this study, we identified and characterized a rolling and erect leaf mutant in rice and named it asrel2. Histological analysis showed that therel2mutant has increased number of bulliform cells and reduced size of middle bulliform cells. We firstly mappedREL2to a 35-kb physical region of chromosome 10 by map-based cloning strategy. Further analysis revealed thatREL2encodes a protein containing DUF630 and DUF632 domains. Inrel2mutant, the mutation of two nucleotide substitutions in DUF630 domain led to the loss-of-function ofREL2locus and the function ofREL2could be confirmed by complementary expression ofREL2inrel2mutant. Further studies showed that REL2 protein is mainly distributed along the plasma membrane of cells and theREL2gene is relatively higher expressed in younger leaves of rice. The results from quantitative RT-PCR analysis indicated thatREL2functioning in the leaf shape formation might have functional linkage with many genes associated with the bulliform cells development, auxin synthesis and transport, etc.ConclusionsREL2 is the DUF domains contained protein which involves in the control of leaf rolling in rice. It is the plasma membrane localization and its functions in the control of leaf morphology might involve in multiple biological processes such as bulliform cell development and auxin synthesis and transport.