New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535
New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535
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鼠伤寒沙门氏菌基因破坏的新方法：鼠伤寒沙门氏菌 TA1535 的 ada 缺失衍生物的构建和表征
作者：
M. Yamada;A. Hakura;T. Sofuni;T. Nohmi
开发了一种破坏鼠伤寒沙门氏菌基因的新方法。该方法的关键步骤是产生末端相容的限制性片段，预连接产生多联体，然后通过电转化进行转化。我们开发并使用这种方法构建了鼠伤寒沙门氏菌TA1535的突变体，其中驻留的ada样（adaST）基因被卡那霉素抗性基因取代，以产生adaST缺失突变体衍生物。与野生型亲本菌株相比，鼠伤寒沙门氏菌adaST缺失菌株在用N-甲基-N'-硝基-N-亚硝基胍处理后并未表现出更高水平的突变性。然而，它确实对 N-甲基-N'-硝基-N-亚硝基胍的杀灭表现出更高的敏感性。讨论了 AdaST 作为转录激活剂的能力。
A new method for gene disruption in Salmonella typhimurium was developed. The key steps of this method are to produce restriction fragments with compatible ends, preligate to produce concatemers, and then transform by electrotransformation. We developed and used this method to construct a mutant of S. typhimurium TA1535 in which the resident ada-like (adaST) gene was replaced with a kanamycin resistance gene to produce an adaST-deletion mutant derivative. The S. typhimurium adaST-deletion strain did not exhibit a higher level of mutability upon treatment with N-methyl-N'-nitro-N-nitrosoguanidine than did its wild-type parent strain. However, it did exhibit a higher sensitivity with respect to killing by N-methyl-N'-nitro-N-nitrosoguanidine. The ability of AdaST to function as a transcriptional activator is discussed.
大肠杆菌中的第二种 DNA 甲基转移酶修复酶。
DOI：
10.1073/pnas.85.9.3039
发表时间：
1988
影响因子：
11.1
作者：
Rebeck,GW;Coons,S;Carroll,P;Samson,L
通讯作者：
Samson,L
ada 转录激活所需的 Ada DNA 修复蛋白区域对于 alkA 的激活不是必需的。
DOI：
10.1073/pnas.88.20.9001
发表时间：
1991
影响因子：
11.1
作者：
Shevell,DE;Walker,GC
通讯作者：
Walker,GC