Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus In vivo.
Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus In vivo.
复制标题
在体内对Epstein-Barr病毒的主要免疫反应期间，抗原特异性CD8+ T细胞的直接可视化。
DOI：
10.1084/jem.187.9.1395
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发表时间：
1998-05-04
期刊：
影响因子：
--
通讯作者：
Rickinson AB
中科院分区：
文献类型：
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作者：
Callan MF;Tan L;Annels N;Ogg GS;Wilson JD;O'Callaghan CA;Steven N;McMichael AJ;Rickinson AB
病毒的初次感染可以刺激细胞毒性 T 细胞的强烈反应。原发性 T 细胞反应中抗原特异性成分与旁观者成分的大小仍然存在争议。在这项研究中，我们使用四聚体主要组织相容性复合物-肽复合物来直接观察人类对 Epstein-Barr 病毒 (EBV) 感染的初次免疫反应期间抗原特异性分化 (CD)8+ T 细胞簇。我们发现，在对该病毒的初次反应期间，会发生活化的抗原特异性 T 细胞的大量扩增。在一个个体中，对单一 EBV 表位具有特异性的 T 细胞占外周血中 CD8+ T 细胞总数的 44%。大多数抗原特异性细胞具有激活/记忆表型，表达人类组织相容性白细胞抗原（HLA）DR、CD38和CD45RO，下调CD62白细胞（CD62L）以及低水平表达CD45RA。从 AIM 恢复后，大多数研究的供体中抗原特异性 T 细胞的频率下降，尽管抗原特异性细胞群在至少 3 年内仍然可以轻松检测到。
Primary infection with virus can stimulate a vigorous cytotoxic T cell response. The magnitude of the antigen-specific component versus the bystander component of a primary T cell response remains controversial. In this study, we have used tetrameric major histocompatibility complex–peptide complexes to directly visualize antigen-specific cluster of differentration (CD)8+ T cells during the primary immune response to Epstein-Barr virus (EBV) infection in humans. We show that massive expansion of activated, antigen-specific T cells occurs during the primary response to this virus. In one individual, T cells specific for a single EBV epitope comprised 44% of the total CD8+ T cells within peripheral blood. The majority of the antigen-specific cells had an activated/memory phenotype, with expression of human histocompatibility leukocyte antigen (HLA) DR, CD38, and CD45RO, downregulation of CD62 leukocyte (CD62L), and low levels of expression of CD45RA. After recovery from AIM, the frequency of antigen-specific T cells fell in most donors studied, although populations of antigen-specific cells continued to be easily detectable for at least 3 yr.