POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2.
POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2.
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POG1 是一种新型酵母基因，可通过 G1 细胞周期蛋白 CLN2 促进信息素停滞的恢复。
DOI：
10.1093/genetics/151.2.531
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Elion,EA
中科院分区：
文献类型：
--
作者：
Leza,MA;Elion,EA
在没有成功的交配，信息素逮捕酿酒酵母细胞重新进入有丝分裂周期，通过一个恢复过程，涉及下调交配丝裂原活化蛋白激酶（MAPK）级联。我们已经分离出一个新的基因，POG 1，其促进恢复平行的MAPK磷酸酶Msg 5。POG 1在过表达时赋予α-因子抗性，并且在msg 5突变体的背景中缺失时增强α-因子敏感性。POG 1的过表达抑制α-因子诱导的G1期阻滞和CLN 1和CLN 2基因的转录抑制。转录抑制的阻断发生在SCB/MCB启动子元件上，其机制需要Bck 1而不是Cln 3。遗传测试强烈认为POG 1通过上调CLN 2基因促进恢复，并且所得的Cln 2蛋白主要通过对Ste 20（交配MAPK级联的激活剂）的作用促进恢复。一个pog 1 cln 3双突变体显示合成突变体表型共享细胞壁完整性和肌动蛋白细胞骨架突变体，没有合成缺陷的表达CLN 1或CLN 2。这些和其他结果表明，POG 1可能在营养生长和恢复过程中调节其他基因。
In the absence of a successful mating, pheromone-arrested Saccharomyces cerevisiae cells reenter the mitotic cycle through a recovery process that involves downregulation of the mating mitogen-activated protein kinase (MAPK) cascade. We have isolated a novel gene, POG1, whose promotion of recovery parallels that of the MAPK phosphatase Msg5. POG1 confers α-factor resistance when overexpressed and enhances α-factor sensitivity when deleted in the background of an msg5 mutant. Overexpression of POG1 inhibits α-factor-induced G1 arrest and transcriptional repression of the CLN1 and CLN2 genes. The block in transcriptional repression occurs at SCB/MCB promoter elements by a mechanism that requires Bck1 but not Cln3. Genetic tests strongly argue that POG1 promotes recovery through upregulation of the CLN2 gene and that the resulting Cln2 protein promotes recovery primarily through an effect on Ste20, an activator of the mating MAPK cascade. A pog1 cln3 double mutant displays synthetic mutant phenotypes shared by cell-wall integrity and actin cytoskeleton mutants, with no synthetic defect in the expression of CLN1 or CLN2. These and other results suggest that POG1 may regulate additional genes during vegetative growth and recovery.