Changes in (3H)-ADTN binding to microsomal and synaptic membrane fractions from rat striata following kainic acid lesions.
Changes in (3H)-ADTN binding to microsomal and synaptic membrane fractions from rat striata following kainic acid lesions.
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红藻氨酸损伤后，(3H)-ADTN 与大鼠纹状体微粒体和突触膜部分结合的变化。
DOI：
10.1016/0006-2952(80)90118-5
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发表时间：
1980
影响因子：
5.8
通讯作者：
S. Freedman
中科院分区：
文献类型：
--
作者：
A. Davis;G. Woodruff;J. Poat;S. Freedman
多巴胺激动剂（3 H）-ADTN的大鼠纹状体和微粒体膜的特异性结合进行了检查。红藻氨酸处理后，高亲和力结合显著降低，腺苷酸环化酶、谷氨酸脱羧酶和胆碱乙酰转移酶的基础水平也显著降低。然而，配体与低亲和力位点的结合增加。这些结果与大鼠纹状体突触膜中高亲和力（3 H）-ADTN结合位点的突触后定位一致。
Specific binding of the dopamine agonist (3H)-ADTN to rat striatal and microsomal membranes was examined. Both high (10 nM) and low (2μM) affinity binding to each membrane fraction was found. Following kainic acid treatment the high affinity binding was significantly reduced, as was the basal level of adenylate cyclase, glutamic acid decarboxylase and choline acetyl transferase. Binding of the ligand to the low affinity site was, however, increased. These results are consistent with a postsynaptic localization of the high affinity (3H)-ADTN binding site in rat striatal synaptic membranes.