4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
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DOI：
10.3791/50450
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发表时间：
2013-08-01
影响因子：
1.2
通讯作者：
Frankel, Gad
中科院分区：
文献类型：
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作者：
Collins, James William;Meganck, Jeffrey A.;Frankel, Gad
该方案概述了使用具有集成mu CT的复合3D漫射光成像断层扫描（DLIT-mu CT）纵向监测生物发光细菌感染所需的步骤，以及随后使用该数据生成感染周期的四维（4D）电影。为了开发4D感染电影并使用IVIS Spectrum CT验证DLIT-mu CT成像用于细菌感染研究，我们使用生物发光C.啮齿类，导致小鼠自限性结肠炎。在这个协议中，我们概述了感染小鼠与生物发光C。通过每天的DLIT-mu CT成像和粪便中的细菌计数，对啮齿类动物的定植进行无创监测，持续8天。IVIS Spectrum CT的使用有助于使用单个成像平台无缝配准光学和mu CT扫描。低剂量mu CT模式能够在感染期间的多个时间点对小鼠进行成像，提供3D生物发光细菌病灶的详细解剖定位，而不会引起累积辐射的伪影。重要的是，感染小鼠的4D电影提供了一个强大的分析工具来监测体内细菌定植动力学。
This protocol outlines the steps required to longitudinally monitor a bioluminescent bacterial infection using composite 3D diffuse light imaging tomography with integrated mu CT (DLIT-mu CT) and the subsequent use of this data to generate a four dimensional (4D) movie of the infection cycle. To develop the 4D infection movies and to validate the DLIT-mu CT imaging for bacterial infection studies using an IVIS Spectrum CT, we used infection with bioluminescent C. rodentium, which causes self-limiting colitis in mice. In this protocol, we outline the infection of mice with bioluminescent C. rodentium and non-invasive monitoring of colonization by daily DLIT-mu CT imaging and bacterial enumeration from feces for 8 days.The use of the IVIS Spectrum CT facilitates seamless co-registration of optical and mu CT scans using a single imaging platform. The low dose mu CT modality enables the imaging of mice at multiple time points during infection, providing detailed anatomical localization of bioluminescent bacterial foci in 3D without causing artifacts from the cumulative radiation. Importantly, the 4D movies of infected mice provide a powerful analytical tool to monitor bacterial colonization dynamics in vivo.