Human Eccrine Sweat Gland Cells Can Reconstitute a Stratified Epidermis
Human Eccrine Sweat Gland Cells Can Reconstitute a Stratified Epidermis
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DOI：
10.1038/jid.2010.83
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发表时间：
2010-08-01
影响因子：
6.5
通讯作者：
Reichmann, Ernst
中科院分区：
文献类型：
--
作者：
Biedermann, Thomas;Pontiggia, Luca;Reichmann, Ernst
小汗腺通常被认为是一种可能的表皮干细胞来源。在这里，我们比较了由表皮角质形成细胞形成的多层上皮和由汗腺细胞形成的多层上皮。我们在体外和体内证明了人汗腺细胞在胶原水凝胶上形成层状毛囊间表皮替代物的能力。结果表明：(1)汗腺细胞形成由10-12个细胞层组成的层状表皮；(2)移植到免疫功能低下大鼠体内后，发育并维持明显的角质层；(3)微丝蛋白、三氯蛋白、总蛋白、包膜蛋白、周斑蛋白、转谷氨酰胺酶I和转谷氨酰胺酶I和III等蛋白质与正常皮肤的模式相匹配；(4)连接复合体和半染色体容易而规则地建立；(5)基底层的细胞增殖达到动态平衡水平；(6)汗腺来源的表皮由血球固定在发达的基膜内；(7)汗腺来源的表皮不表达掌底或粘膜标志物。这些数据表明，人类的汗腺是角质形成细胞的另一个来源，角质形成细胞可以产生分层的表皮。我们的发现提出了一个问题，即人类皮肤在多大程度上被外分泌汗腺细胞修复和/或永久更新。
Eccrine sweat glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine sweat gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine sweat gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10-12 cell layers is formed by sweat gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the sweat gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the sweat gland-derived epidermis. These data suggest that human eccrine sweat glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine sweat gland cells.