Comparative Study of Transcriptome Profiles of Mouse Livers and Skins Infected by Fork-Tailed or Non-Fork-Tailed Schistosoma japonicum.
Comparative Study of Transcriptome Profiles of Mouse Livers and Skins Infected by Fork-Tailed or Non-Fork-Tailed Schistosoma japonicum.
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叉尾与非叉尾日本血吸虫感染小鼠肝脏和皮肤转录组谱的比较研究
DOI：
10.3389/fmicb.2017.01648
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zou FC
中科院分区：
文献类型：
--
作者：
Yang Y;He JJ;Hu S;Chang H;Xiang X;Yang JF;Zou FC
日本血吸虫（S. japonicum）是一种世界范围内广泛传播的病原体，它能穿透宿主皮肤，在被感染的宿主体内引起纤维化、肉芽肿形成、肝硬化和肝肿大等多种疾病。本研究首次采用RNA-seq方法分析了感染日本血吸虫分叉尾尾蚴和非分叉尾尾蚴的小鼠肝脏和皮肤的转录组学特征。本研究结果表明，感染叉尾日本血吸虫尾蚴和非叉尾日本血吸虫尾蚴的肝脏和皮肤的转录组景观不同。日本血吸虫对肝脏代谢过程有很大的影响。叉尾血吸虫尾蚴上调肝脏代谢过程，而非叉尾血吸虫尾蚴下调肝脏代谢过程。无论日本血吸虫尾蚴的生物类型如何，宿主的代谢过程或代谢酶的表达变化都可能改变宿主的药代动力学。感染后皮肤的变化主要表现为免疫反应的上调。在日本血吸虫皮肤感染期间，与未感染日本血吸虫尾蚴相比，感染日本血吸虫尾蚴引起的免疫应答更强。转录因子富集分析表明，Irf7、Stat1和Stat2可能在日本血吸虫分叉尾尾蚴感染过程中发挥重要的基因调控作用。
Schistosoma japonicum (S. japonicum) is a worldwide spread pathogen which penetrates host skin and then induces several diseases in infected host, such as fibrosis, formation of granulomas, hepatocirrhosis, and hepatomegaly. In present study, for the first time, transcriptomic profiles of mouse livers and skins infected by fork-tailed S. japonicum cercaria or non-fork-tailed S. japonicum cercaria were analyzed by using RNA-seq. The present findings demonstrated that transcriptomic landscapes of livers and skins infected by fork-tailed S. japonicum cercaria or non-fork-tailed S. japonicum cercaria were different. S. japonicum has great influence on hepatic metabolic processes. Fork-tailed S. japonicum cercaria upregulated hepatic metabolic processes, while non-fork-tailed S. japonicum cercaria downregulated hepatic metabolic processes. For the metabolism process or the metabolism enzyme expressional change, the pharmacokinetics of host could be changed during S. japonicum infection, regardless the biotypes of S. japonicum cercariae. The changes of infected skins focused on upregulation of immune response. During the S. japonicum skin infection period, fork-tailed S. japonicum cercaria infection induced stronger immune response comparing with that immune response triggered by non-fork-tailed S. japonicum cercaria. The transcription factor enrichment analysis showed that Irf7, Stat1 and Stat2 could play important roles in gene regulation during fork-tailed S. japonicum cercaria infection.