Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote-an immunocytochemical approach adapted to single cells
Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote-an immunocytochemical approach adapted to single cells
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DOI：
10.1007/s00497-004-0221-0
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Kranz, E
中科院分区：
文献类型：
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作者：
Hoshino, Y;Scholten, S;Kranz, E
通过使用单细胞显微操作技术，我们开发了一种免疫细胞化学程序来检查分离和培养细胞中的亚细胞蛋白质定位。使用抗α微管蛋白抗体在分离的单个卵细胞和正在发育的玉米受精卵中检查微管的定位。在卵细胞中，检测到一些皮质微管，但很少观察到组织良好的微管。相反，在发育中的受精卵中观察到明显的皮质微管和从细胞核辐射到细胞外周的细胞质微管链。体外受精后 7 小时内，仅在受精卵中观察到皮质微管。此后，辐射微管又出现，并在受精卵发育过程中持续存在。这些结果表明，受精诱导的玉米受精卵细胞中微管组织发生了早期且明显的变化。
By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti-alpha-tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.