Dielectrophoretic segregation of different human cell types on microscope slides
Dielectrophoretic segregation of different human cell types on microscope slides
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DOI：
10.1021/ac048196z
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发表时间：
2005-05-01
影响因子：
7.4
通讯作者：
Gascoyne, PRC
中科院分区：
文献类型：
--
作者：
Das, CM;Becker, F;Gascoyne, PRC
提出了一种制备显微镜检查用细胞的新方法，其中细胞混合物通过介电电泳力分离，并同时收集到载玻片上的特征区域中。该方法将细胞直接从悬浮培养基捕获到载玻片上，减少细胞损失。此外，它还利用了细胞介电特性的差异，这敏感地反映了它们的形态。由于不同的细胞类型被捕获在载玻片上的特征区域中，因此该技术代表了现有载玻片制备方法的进步，例如细胞随机分布的离心和涂片。特别是，新方法应该有助于检测罕见和异常的细胞亚群，否则这些细胞亚群在正常细胞的高背景下可能会被忽视。除了适用于传统的显微镜检查和自动载玻片扫描方法外，它还与组织化学和免疫化学技术以及新兴的分子和蛋白质组学方法兼容。本文介绍了这种所谓的电涂片仪器的原理和设计，并显示实验结果，验证了模型细胞系和正常外周血亚群的方法的理论和适用性。
A new method for preparing cells for microscopic examination is presented in which cell mixtures are fractionated by dielectrophoretic forces and simultaneously collected into characteristic zones on slides. The method traps cells directly from the suspending medium onto the slide, reducing cell loss. Furthermore, it exploits differences in the dielectric properties of the cells, which sensitively reflect their morphology. Because different cell types are trapped in characteristic zones on the slide, the technique represents an advance over existing methods for slide preparation, such as centrifugation and smears where cells are randomly distributed. In particular, the new method should aid in the detection of rare and anomalous cell subpopulations that might otherwise go unnoticed against a high background of normal cells. As well as being suitable for traditional microscopic examination and automated slide scanning approaches, it is compatible with histochemical and immunochemical techniques, as well as emerging molecular and proteomic methods. This paper describes the rationale and design of this so-called electrosmear instrumentation and shows experimental results that verify the theory and applicability of the method with model cell lines and normal peripheral blood subpopulations.