Glucosinolate biosynthetic genes in Brassica rapa
Glucosinolate biosynthetic genes in Brassica rapa
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DOI：
10.1016/j.gene.2011.07.021
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发表时间：
2011-11-10
期刊：
影响因子：
3.5
通讯作者：
Wang, Xiaowu
中科院分区：
文献类型：
--
作者：
Wang, Hui;Wu, Jian;Wang, Xiaowu
硫代葡萄糖苷（GS）是十字花科植物中广泛存在的一类氨基酸衍生的次生代谢产物。硫代葡萄糖苷及其降解产物在病原体和昆虫相互作用以及人类健康中起着重要作用。为了阐明芜菁中硫代葡萄糖甙的生物合成途径，我们对拟南芥和B. B进行了比较基因组分析。在基因组水平上。我们在B中鉴定了102个推定基因。rapa是A. thaliana.除一个基因外，所有基因都成功地定位在10条染色体上。大多数GS基因在B中以多个拷贝存在。拉帕。硫甙生物合成途径与A. thaliana和B.强奸罪也成立。B中同源的GS基因。rapa和A.拟南芥GS基因的同源性为59-91%，93%的GS基因在B之间具有同线性。rapa和A. thaliana.此外，还介绍了B. rapa GS（BrGS）基因与已知的B的进化趋异相一致。这可能有助于解释B中GS的分布和积累。拉帕。(C)2011 Elsevier B. V.保留所有权利。
Glucosinolates (GS) are a group of amino acid-derived secondary metabolites found throughout the Cruciferae family. Glucosinolates and their degradation products play important roles in pathogen and insect interactions, as well as in human health. In order to elucidate the glucosinolate biosynthetic pathway in Brassica rapa, we conducted comparative genomic analyses of Arabidopsis thaliana and B. rapa on a genome-wide level. We identified 102 putative genes in B. rapa as the orthologs of 52 GS genes in A. thaliana. All but one gene was successfully mapped on 10 chromosomes. Most GS genes exist in more than one copy in B. rapa. A high co-linearity in the glucosinolate biosynthetic pathway between A. thaliana and B. rape was also established. The homologous GS genes in B. rapa and A. thaliana share 59-91% nucleotide sequence identity and 93% of the GS genes exhibit synteny between B. rapa and A. thaliana. Moreover, the structure and arrangement of the B. rapa GS (BrGS) genes correspond with the known evolutionary divergence of B. rapa, and may help explain the profiles and accumulation of GS in B. rapa. (C) 2011 Elsevier B.V. All rights reserved.