Tissue engineering of recellularized small-diameter vascular grafts
Tissue engineering of recellularized small-diameter vascular grafts
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DOI：
10.1089/ten.2005.11.778
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发表时间：
2005-05-01
影响因子：
--
通讯作者：
Brown, DL
中科院分区：
文献类型：
--
作者：
Borschel, GH;Huang, YC;Brown, DL
组织工程化的小直径动脉移植物将有利于需要血管重建手术的患者。我们的目标是生产一种组织工程血管移植物，具有高通畅率，可以承受动脉压力。用一系列洗涤剂溶液使大鼠动脉细胞化，通过与内皮细胞的原代培养物孵育再细胞化，并作为插入移植物植入股总动脉中。将未再细胞化的脱细胞移植物植入另一组对照动物中。未给予全身抗凝剂。移植物在4周时进行明确的通畅性评价和组织学检查; 89%的再细胞化移植物和29%的对照移植物保持通畅。弹性蛋白染色显示植入前脱细胞移植物的中膜内保留了弹性纤维。移植物的免疫组织化学染色显示，在保持通畅的移植物中，管腔表面上有完整的内皮细胞层。观察到平滑肌细胞重新填充血管壁。基质的机械性能与自体血管相当。这样的策略可以提供用于血管旁路手术的小血管自体收获的替代方案。
A tissue-engineered small-diameter arterial graft would be of benefit to patients requiring vascular reconstructive procedures. Our objective was to produce a tissue-engineered vascular graft with a high patency rate that could withstand arterial pressures. Rat arteries were a cellularized with a series of detergent solutions, recellularized by incubation with a primary culture of endothelial cells, and implanted as interposition grafts in the common femoral artery. Acellular grafts that had not been recellularized were implanted in a separate group of control animals. No systemic anticoagulants were administered. Grafts were explanted at 4 weeks for definitive patency evaluation and histologic examination; 89% of the recellularized grafts and 29% of the control grafts remained patent. Elastin staining demonstrated the preservation of elastic fibers within the media of the acellular grafts before implantation. Immunohistochemical staining of explanted grafts demonstrated a complete layer of endothelial cells on the lumenal surface in grafts that remained patent. Smooth muscle cells were observed to have repopulated the vessel walls. The mechanical properties of the matrix were comparable to native vessels. Such a strategy may present an alternative to autologous harvest of small vessels for use in vascular bypass procedures.