Discharge patterning in rat olfactory bulb mitral cells in vivo.
Discharge patterning in rat olfactory bulb mitral cells in vivo.
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DOI：
10.14814/phy2.12021
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发表时间：
2014-10-01
影响因子：
2.5
通讯作者：
Ludwig M
中科院分区：
文献类型：
--
作者：
Leng G;Hashimoto H;Tsuji C;Sabatier N;Ludwig M
本文对氨基甲酸乙酯麻醉大鼠主嗅球二尖瓣细胞的放电特性进行了详细的统计分析。从二尖瓣细胞层记录神经元，并通过施加于外侧嗅道的刺激进行反向识别。所有二尖瓣细胞都表现出重复的、长时间的动作电位爆发，通常持续100 ~ 100秒，并以同样长的间隔分开；大约一半的人在爆发之间完全沉默。在靠近二尖瓣细胞的非二尖瓣细胞中没有观察到这种破裂。即使在相邻的二尖瓣细胞中，爆发也是不同步的。大多数二尖瓣细胞的突内活动对自发性呼吸节律有强烈的牵引作用；类似的夹带在一些非二尖瓣细胞中可见，但并非全部。所有二尖瓣细胞在尖峰后约25 msec出现兴奋性峰值，反映在尖峰间隔分布和相应的危险函数中。大约一半的人也在6毫秒左右出现峰值，反映了双重尖峰的常见现象。非二尖瓣细胞则没有这种双重尖峰。爆发的强度通常在爆发的前20-30秒增加，在此期间很少或没有双重爆发。在20-30秒后（在表现出双重的细胞中），只要爆发持续，就会频繁地出现双重，最多可达10个双重。最后一次双重反应之后是一个延长的相对不应期，其持续时间与列车长度无关。在被特定气味刺激的细胞中，在爆发之间的沉默时期，反应性明显比爆发期间更强。相反，在被特定气味抑制的细胞中，反应性只有在细胞活跃时才明显。来自单元的大量原始（事件计时）数据以及这些分析的详细信息作为补充材料提供，可供其他人免费使用。本文对氨基甲酸乙酯麻醉大鼠主嗅球二尖瓣细胞的放电特性进行了详细的统计分析。来自单元的大量原始（事件计时）数据以及这些分析的详细信息作为补充材料提供，可供其他人免费使用。
Here we present a detailed statistical analysis of the discharge characteristics of mitral cells of the main olfactory bulb of urethane‐anesthetized rats. Neurons were recorded from the mitral cell layer, and antidromically identified by stimuli applied to the lateral olfactory tract. All mitral cells displayed repeated, prolonged bursts of action potentials typically lasting >100 sec and separated by similarly long intervals; about half were completely silent between bursts. No such bursting was observed in nonmitral cells recorded in close proximity to mitral cells. Bursts were asynchronous among even adjacent mitral cells. The intraburst activity of most mitral cells showed strong entrainment to the spontaneous respiratory rhythm; similar entrainment was seen in some, but not all nonmitral cells. All mitral cells displayed a peak of excitability at ~25 msec after spikes, as reflected by a peak in the interspike interval distribution and in the corresponding hazard function. About half also showed a peak at about 6 msec, reflecting the common occurrence of doublet spikes. Nonmitral cells showed no such doublet spikes. Bursts typically increased in intensity over the first 20–30 sec of a burst, during which time doublets were rare or absent. After 20–30 sec (in cells that exhibited doublets), doublets occurred frequently for as long as the burst persisted, in trains of up to 10 doublets. The last doublet was followed by an extended relative refractory period the duration of which was independent of train length. In cells that were excited by application of a particular odor, responsiveness was apparently greater during silent periods between bursts than during bursts. Conversely in cells that were inhibited by a particular odor, responsiveness was only apparent when cells were active. Extensive raw (event timing) data from the cells, together with details of those analyses, are provided as supplementary material, freely available for secondary use by others. Here we present a detailed statistical analysis of the discharge characteristics of mitral cells of the main olfactory bulb of urethane‐anesthetized rats. Extensive raw (event timing) data from the cells, together with details of those analyses, are provided as supplementary material, freely available for secondary use by others.