Demonstration of the production of frameshift and base-substitution mutations by quasipalindromic DNA sequences.
Demonstration of the production of frameshift and base-substitution mutations by quasipalindromic DNA sequences.
复制标题
演示通过准回文 DNA 序列产生移码和碱基取代突变。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
L. S. Ripley
中科院分区：
文献类型：
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作者：
J. D. de Boer;L. S. Ripley
体内生产的移码和碱基取代突变预测的结果的代谢处理的错配准回文DNA序列已被证实。自发移码突变的T4 rII基因已被遗传映射到准回文DNA序列进行测序。一些突变序列正是那些预测的突变模型的基础上错位准回文。此外，这些序列是不同的经典移码模型的基础上错配重复序列的预测。这里报告的rII移码突变体序列是由于缺失了一个或多个特定的碱基，如果准回文素呈现发夹二级结构，这些碱基将保持环状。一个发夹不仅预测了两个碱基的缺失（移码），而且还预测了附近但不连续的碱基取代的伴随产生。预测多达三个碱基的取代以及移码是回文中单个突变事件的结果。两个独立的预测删除移码的例子中发现的小样本的测序自发移码检查，都与预测的颠换和过渡碱基取代。
The in vivo production of frameshift and base-substitution mutations predicted as a consequence of the metabolic processing of misaligned quasipalindromic DNA sequences has been confirmed. Spontaneous frameshift mutations of the T4 rII gene that had been genetically mapped to quasipalindromic DNA sequences were sequenced. Some of the mutant sequences are exactly those predicted by a mutational model based on misaligned quasipalindromes. Furthermore, these sequences are distinct from those predicted by the classical frameshift model based on misaligned repeated sequences. The rII frameshift mutant sequences reported here result from the deletion of a specific base or bases that would remain looped out should the quasipalindromes assume a hairpin secondary structure. One hairpin predicted not only the deletion of two bases (a frameshift) but the concomitant production of nearby but noncontiguous base substitutions. The substitution of as many as three bases as well as the frameshift were predicted to arise as a consequence of a single mutational event in the palindrome. Two independent examples of the predicted deletion frameshift were found among the small sample of sequenced spontaneous frameshifts examined and both were associated with the predicted transversion and transition base substitutions.