Localization of choline acetyltransferase‐like immunoreactivity in the embryonic chick retina
Localization of choline acetyltransferase‐like immunoreactivity in the embryonic chick retina
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胚胎鸡视网膜中胆碱乙酰转移酶样免疫反应的定位
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
I. Morgan
中科院分区：
文献类型：
--
作者：
A. Spira;T. J. Millar;I. Ishimoto;M. Epstein;Carl D. Johnson;J. Dahl;I. Morgan
用胆碱乙酰转移酶抗血清对胚胎和孵化后小鸡视网膜中乙酰胆碱合成的假定位点进行化学定位;将所得胆碱乙酰转移酶样免疫反应性（ChAT-IR）与已证实的乙酰转移酶（AChE）活性位点进行比较，并跟踪发育过程中定位的变化。结果证实了早期和快速的发展过程中所描述的小鸡的视网膜胆碱能系统在以前的生化和形态学研究。在胚胎第6.5天，在靠近视网膜玻璃体表面的细胞中首次检测到免疫反应性。到第8天，它存在于两个并列的行中的细胞中;到第9天，两行分离，免疫反应性在内部网状层的两个亚层中是明显的。在第10天，分布类似于在后盖鸡中的分布，即在内核层中的I型胆碱能细胞和在神经节细胞层中的II型细胞中的分布（Millar等人：Neurosci. Lett. 61：311-316，'85.），与大多数脊椎动物相似。孵化前三天，第三人口的弱免疫反应细胞（III型细胞）内出现的内核层。可定位的ChAT‐IR的发作发生在无长突细胞及其突起中，在突触发生期之前。乙酰胆碱酯酶活性定位于比ChAT-IR更早的年龄，并且在所有年龄都存在于更多的细胞中。所获得的结果支持这样的观点，即“位移”胆碱能无长突细胞开始分化在同一时间，并在同一视网膜区域作为I型胆碱能细胞。这两个群体的分离是无长突细胞和神经节细胞的分支过程的结果，而不是细胞在层之间的二次迁移的结果。
Putative sites of acetylcholine synthesis in the retina of the embryonic and posthatched chick were localized immunohistochemically with antisera to choline acetyltransferase; the resultant choline acetyltransferase‐like immunoreactivity (ChAT‐IR) was compared to demonstrated sites of acetyltransferase (AChE) activity, and changes were followed in localization during development. The results confirmed the early and rapid course of development of the chick's retinal cholinergic system described in previous biochemical and morphological studies. Immunoreactivity was first detected at embryonic day 6.5 in cells close to the retina's vitreal surface. By 8 days it was present in cells in two juxtaposed rows; by the ninth day the two rows were separated and Immunoreactivity was evident in two sublaminae of the inner plexiform layer. On the tenth day distribution was like that in the posthatched chicken, in type I cholinergic cells in the inner nuclear layer and in type II cells in the ganglion cell layer (Millar et al.: Neurosci. Lett. 61:311–316, '85.), and similar to that of most vertebrates. Three days before hatching, a third population of weakly immunoreactive cells (type III cells) appeared within the inner nuclear layer. The onset of localizable ChAT‐IR occurred in amacrine cells and in their processes, before the period of synaptogenesis. Acetylcholinesterase activity was localized at an earlier age than ChAT‐IR, and at all ages was present in more cells. The results obtained support the view that “displaced” cholinergic amacrine cells begin to differentiate at the same time and in the same retinal region as type I cholinergic cells. Separation of the two groups is a consequence of the ramification of processes of amacrine and ganglion cells rather than a result of the secondary migration of cells between layers.
突触发生过程中毒蕈碱乙酰胆碱受体系统的分子改变。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Large,TH;Cho,NJ;DeMello,FG;Klein,WL
通讯作者：
Klein,WL
产后兔视网膜中多巴胺能神经元的个体发生：突触前和突触后元件。
影响因子：
2.9
作者：
Parkinson,D;Rando,RR
通讯作者：
Rando,RR