Functional heterogeneity of macrophages: subclasses of peritoneal macrophages with different antigen-binding activities and immune complex receptors.
Functional heterogeneity of macrophages: subclasses of peritoneal macrophages with different antigen-binding activities and immune complex receptors.
复制标题
巨噬细胞的功能异质性：具有不同抗原结合活性和免疫复合物受体的腹膜巨噬细胞亚类。
作者：
W. Walker
从兔腹膜渗出液粘附细胞的24小时培养物制备的细胞悬液在Ficoll的不连续梯度上被分离成密度亚类。在获得的五个亚类中，巨噬细胞在两个密度最小的亚类中占95%以上的细胞，在中等密度的亚类中占90%以上的细胞。最密集的亚类含有约80%的巨噬细胞和20%的淋巴细胞。
在添加抗体的存在和不存在下，用选定数量的125 I标记的抗原研究了亚类的抗原结合特性。在不存在抗体的情况下，这些亚类的抗原结合活性彼此不同;这些差异与测试抗原和抗原聚集状态无关。在特异性抗体的存在下，两个巨噬细胞亚类比其他亚类结合更多的抗原。通过研究细胞与致敏鸡红细胞孵育时形成红细胞的能力，证实了这些亚类的增强反应。结果表明，这两个亚类中的巨噬细胞在免疫球蛋白受体位点方面与其他巨噬细胞亚类定量和/或定性不同。
这些研究证明了使用梯度分离程序获得富含巨噬细胞的功能不同的细胞亚类的可行性。明确定义的巨噬细胞群体的可用性应允许更精确的研究巨噬细胞的免疫功能。
Cell suspensions prepared from 24-hour cultures of adherent peritoneal exudate cells from rabbits were separated into density subclasses on discontinuous gradients of Ficoll. Of the five subclasses obtained, macrophages comprised over 95 per cent of the cells in the two least dense subclasses and over 90 per cent of the cells in the subclasses of intermediate density. The most dense subclass contained approximately 80 per cent macrophages and 20 per cent lymphocytes.
The antigen-binding properties of the subclasses were studied with a selected number of 125I-labelled antigens in the presence and absence of added antibodies. In the absence of antibody the subclasses differed from one another in antigen-binding activities; these differences were independent of both the test antigens and the state of antigen aggregation. In the presence of specific antibody, two macrophage subclasses bound substantially more antigen than the other subclasses. The enhanced responses of these subclasses were confirmed by studying the cells' capacity to form rosettes when incubated with sensitized chicken erythrocytes. The results indicated that macrophages in these two subclasses differed quantitatively and/or qualitatively from other macrophage subclasses with regard to immunoglobulin receptor sites.
These studies demonstrate the feasibility of using gradient separation procedures to obtain functionally distinct subclasses of cells rich in macrophages. The availability of well-defined macrophage populations should permit more precise studies of macrophage functions in immunity.