A retrovirus isolated from cell lines derived from neurofibromas in bicolor damselfish (Pomacentrus partitus).
A retrovirus isolated from cell lines derived from neurofibromas in bicolor damselfish (Pomacentrus partitus).
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从双色雀鲷 (Pomacentrus partitus) 神经纤维瘤细胞系中分离出的逆转录病毒。
DOI：
10.1099/0022-1317-77-6-1181
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Gill,KA
中科院分区：
文献类型：
--
作者：
Schmale,MC;Aman,MR;Gill,KA
雀鲷神经纤维瘤病（DNF）是一种自然发生的肿瘤性疾病，影响生活在美国佛罗里达南部珊瑚礁上的双色雀鲷（Pomacentrus partitus）。该疾病包括多发性神经纤维瘤、神经纤维肉瘤和色素瘤，并已被提议作为人类1型神经纤维瘤病的动物模型。DNF可通过注射肿瘤匀浆粗品、匀浆的无细胞培养物或来自肿瘤细胞系的细胞而传播。对来自自发或实验诱导的DNF的鱼的致瘤细胞系的分析显示，病毒颗粒从细胞出芽并存在于条件培养基中。90-110 nm的颗粒类似于C型逆转录病毒。该病毒在蔗糖中的浮力密度为1.14-1.17 g/cm ~ 2，至少有6种15 - 80 kDa的病毒蛋白和逆转录酶（RT）活性。用poly（rC）、oligo（dG）模板、引物组合和浓度为0.5- 1.0mM的Mn 2+使RT活性最大化。RT的最佳温度被确定为20 ℃，这一发现与该物种所遇到的环境温度一致。这种逆转录病毒，暂命名为雀鲷神经纤维瘤病病毒（DNF V），可能是DNF的病原体。无论是DNFV还是另一种尚未鉴定的病毒是DNF的原因，这种因子在病毒肿瘤发生中可能是独特的;涉及DNF的细胞类型的肿瘤转化，Schwann细胞和色素细胞，在任何其他传染性肿瘤中都没有记录。
Damselfish neurofibromatosis (DNF) is a naturally occurring, neoplastic disease affecting bicolor damselfish (Pomacentrus partitus) living on coral reefs in southern Florida, USA. The disease consists of multiple neurofibromas, neurofibrosarcomas and chromatophoromas and has been proposed as an animal model for neurofibromatosis type 1 in humans. DNF is transmissible by injection of crude tumour homogenates, cell-free filtrates of homogenates or cells from tumour cell lines. An analysis of tumorigenic cell lines derived from fish with spontaneous or experimentally induced DNF revealed virus particles budding from cells and present in conditioned media. The 90–110 nm particles resembled type C retroviruses. This virus exhibited a buoyant density of 1.14–1.17 g/cm2in sucrose, at least six virus proteins of 15 to 80 kDa and reverse transcriptase (RT) activity. RT activity was maximized with a poly(rC).oligo(dG) template.primer combination and Mn2+at a concentration of 0.5–1.0 mm. The optimum temperature for RT was determined to be 20 °C, a finding consistent with the ambient temperatures encountered by this species. This retrovirus, tentatively named damselfish neurofibromatosis virus (DNFV) may be the aetiological agent of DNF. Whether DNFV or another, as yet unidentified, virus is the cause of DNF, this agent may be unique in virus oncogenesis; neoplastic transformation of the cell types involved in DNF, Schwann cells and chromatophores, has not been documented in any other transmissible tumour.