Microwave pretreatment for enzymatic saccharification of sweet sorghum bagasse
Microwave pretreatment for enzymatic saccharification of sweet sorghum bagasse
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DOI：
10.1016/j.biombioe.2012.01.006
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发表时间：
2012-04-01
影响因子：
6
通讯作者：
Markevicius, Gediminas
中科院分区：
文献类型：
--
作者：
Choudhary, Ruplal;Umagiliyage, Arosha Loku;Markevicius, Gediminas
研究了四种石灰剂量（0、0.1、0.15和0.2 g/g甘蔗渣）、两种含水量（10或20 ml/g甘蔗渣）和三种暴露时间（2、4和6 min）对甜高粱甘蔗渣（SSB）的微波辐射预处理效果。确定最佳预处理条件为0.1 g石灰和10 ml水/ g SSB （4 mm）。在此条件下，糖产量为32.2 g/100 g SSB（相当于最大潜能糖的52.6%）。在相同含水量和暴露时间下，不加石灰，糖产量为39.8 g/100 g SSB（相当于最大总糖的65.1%）。不加石灰的高糖回收率主要是由于预处理过程中糖的高释放。但添加石灰时，会发生糖的降解，导致糖的产量减少，尽管石灰确实使纤维素更容易被酶接近，这可以从酶水解过程中总还原糖的增加百分比较高中得到证明。本研究的结果得到了FTIR和SEM图像的有力支持。总之，在很短的时间和简单的设置下，微波辐射显示出很大的希望成为一种领先的SSB预处理技术。(C) 2012 Elsevier Ltd.版权所有。
Pretreatment of sweet sorghum bagasse (SSB) through microwave radiation was evaluated at four lime doses (0, 0.1, 0.15, and 0.2 g/g SSB), two water content of 10 or 20 ml/g SSB, and three exposure times as 2, 4, and 6 min. Optimal pretreatment condition was identified as 0.1 g lime and 10 ml water per g SSB in 4 mm. Under this condition, sugar yield of 32.2 g/100 g SSB (equivalent to 52.6% of maximal potential sugars) was achieved. With the same water content and exposure time, but without lime, sugar yield of 39.8 g/100 g SSB (equivalent to 65.1% of maximal total sugars) was observed. The higher sugar recovery without lime was mainly due to high sugar release during pretreatment. But with lime, sugar degradation took place, which resulted in less sugar yield though lime did make cellulose more accessible to enzymes as evidenced by higher percentage of increase of total reducing sugars during enzymatic hydrolysis. Results from this study were strongly supported by FTIR and SEM images. Overall, in a very short time and simple setup, microwave radiation shows great promise to be a leading pretreatment technique for SSB. (C) 2012 Elsevier Ltd. All rights reserved.