Neuropeptide Autoantibodies Assay
Neuropeptide Autoantibodies Assay
复制标题
DOI:
10.1007/978-1-61779-310-3_19
复制
发表时间:
2011-01-01
期刊:
影响因子:
--
通讯作者:
Fetissov, Serguei O.
中科院分区:
文献类型:
--
作者:
Fetissov, Serguei O.
This protocol describes an ELISA-based method for assaying serum levels of autoantibodies reactive with neuropeptides. The method allows for measuring relative amounts of free and bound, i.e., those present in immune complexes, autoantibodies using two types of sample buffers providing normal and dissociative conditions, respectively. This method can be applied to measure autoantibody levels directed against other than neuropeptide molecules and in a variety of biological fluids.