Neuropeptide Autoantibodies Assay

Neuropeptide Autoantibodies Assay
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DOI:
10.1007/978-1-61779-310-3_19
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发表时间:
2011-01-01
期刊:
NEUROPEPTIDES: METHODS AND PROTOCOLS
影响因子:
--
通讯作者:
Fetissov, Serguei O.
Fetissov, Serguei O.
中科院分区:
其他
文献类型:
--
作者:
Fetissov, Serguei O.

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该方案描述了一种基于ELISA的方法,用于测定与神经肽反应的自身抗体的血清水平。该方法允许测量游离和结合的相对量,即,存在于免疫复合物中的那些,使用分别提供正常和解离条件的两种类型的样品缓冲液的自身抗体。该方法可应用于测量针对神经肽分子以外的自身抗体水平和各种生物液体中的自身抗体水平。
This protocol describes an ELISA-based method for assaying serum levels of autoantibodies reactive with neuropeptides. The method allows for measuring relative amounts of free and bound, i.e., those present in immune complexes, autoantibodies using two types of sample buffers providing normal and dissociative conditions, respectively. This method can be applied to measure autoantibody levels directed against other than neuropeptide molecules and in a variety of biological fluids.