Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
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通过修改 Berk-Sharp 程序进行 RNA 作图:15 S β-珠蛋白 mRNA 前体和成熟 10 s β-珠蛋白 mRNA 的 5 末端具有相同的图谱坐标。

DOI:
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发表时间:
1979
影响因子:
14.9
通讯作者:
C. Weissmann
C. Weissmann
中科院分区:
生物学2区
文献类型:
--
作者:
Robert F. Weaver;C. Weissmann

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我们使用了一种改进的Berk-Sharp技术来确定小鼠15s β -珠蛋白前体的5'末端和成熟mRNA具有相同的图谱坐标。修饰包括使用5'(或3')末端标记探针;它允许在存在过量成熟mRNA的情况下检测前体。
We have used a modification of the Berk-Sharp technique to determine that the 5' termini of the mouse 15 S beta-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5' (or 3') terminally labeled probes; it allows the detection of the precursor in the presence of excess mature mRNA.