Boronate Based Metal-Free Platform for Diphosphate-Specific Molecular Recognitions
Boronate Based Metal-Free Platform for Diphosphate-Specific Molecular Recognitions
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DOI：
10.1021/ol5036003
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发表时间：
2015-02-06
期刊：
影响因子：
5.2
通讯作者：
Miyahara, Yuji
中科院分区：
文献类型：
--
作者：
Sanjoh, Mai;Iizuka, Daisuke;Miyahara, Yuji
可逆的硼酸-二醇相互作用为分子识别提供了一个多功能的合成平台，其结合特异性可以在分子上定制。我们发现，在弱酸性条件下，酸度相对较强的硼酸盐衍生物通常会在其他磷酸盐中进行二磷酸盐特异性识别，这是一个与DNA测序相关的特征。B-11和P-31核磁共振研究发现，“四面体硼酸盐和二价二磷酸”是一对负责形成1:1的化学计量络合物，这表现出独特的ph依赖性稳定性。
A reversible boronate-diol interaction provides a versatile synthetic platform for molecular recognitions whose binding specificity can be molecularly tailored. We found that boronate derivatives with relatively strong acidity generally undergo a diphosphate-specific recognition among other phosphates under weakly acidic pH conditions, a feature relevant to DNA sequencing. B-11 and P-31 NMR studies identified "tetrahedral boronate and divalent diphosphate" as a pair responsible for forming a 1:1 stoichiometric complex, which manifests as a unique pH-dependent stability.