Zymosan promotes proliferation,Candida albicansadhesion and IL-1β production of oral squamous cell carcinoma in vitro
Zymosan promotes proliferation,Candida albicansadhesion and IL-1β production of oral squamous cell carcinoma in vitro
复制标题
酵母聚糖促进口腔鳞状细胞癌体外增殖、白色念珠菌粘附和IL-1β产生
DOI：
10.1186/s13027-020-00315-6
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发表时间：
2020-07-31
影响因子：
3.7
通讯作者：
Chen, Fuxiang
中科院分区：
文献类型：
--
作者：
Chen, Xu;Luo, Qingqiong;Chen, Fuxiang
口腔鳞状细胞癌（OSCC）是头颈部鳞状细胞癌（HNSCC）中最常见的类型，酵母多糖（ZYM）是酵母细胞壁的一种成分，其对口腔癌的影响尚不清楚。采用CCK-8法检测ZYM对口腔鳞癌细胞株WSU-HN 4、WSU-HN 6和CAL 27增殖的影响，并通过实时荧光定量PCR、免疫荧光和western blot等方法探讨ZYM的作用机制。采用细胞粘附实验检测白念珠菌对口腔鳞癌细胞的粘附作用，并检测相关基因TLR 2、MyD 88、NLRP 3、ASC、Caspase-1、IL-1 β和蛋白质TLR 2、MyD 88、NF-κ B p65、p-NF-κ B p65、E-cadherin的表达。此外，使用化学发光免疫分析法（CLIA）检测OSCC细胞产生的促炎细胞因子包括IL-6、IL-8、TNF-α和IL-1 β。CCK-8法显示ZYM通过TLR 2/MyD 88途径促进WSU-HN 4、WSU-HN 6和CAL 27细胞增殖。细胞粘附实验表明，C.与对照组相比，ZYM处理的OSCC细胞中每个视野的白色细胞显著增加。当用ZYM处理时，OSCC细胞分泌显著更多的促炎细胞因子IL-1 β，这可以增强口腔癌微环境中的炎症。综上所述，真菌细胞壁中的ZYM促进了C.体外实验表明，口腔鳞癌中白细胞粘附和IL-1 β的产生。
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC), and the effect of zymosan (ZYM), a component of the yeast cell wall, on oral cancer remains unclear. The CCK-8 proliferation assay was performed to evaluate the effect of ZYM on the proliferation of the OSCC cell lines WSU-HN4, WSU-HN6 and CAL27, and the potential mechanism was explored by quantitative real-time PCR, immunofluorescence assay and western blot. A cell adhesion assay was conducted to determine the adhesion ofCandida albicansto OSCC cells, and the expression of related genes, includingTLR2,MyD88,NLRP3,ASC,Caspase-1andIL-1 beta, and proteins, including TLR2, MyD88, NF-kappa B p65, p-NF-kappa B p65 and E-cadherin was determined. Additionally, the pro-inflammatory cytokines including IL-6, IL-8, TNF-alpha and IL-1 beta produced by OSCC cells were detected using a chemiluminescence immunoassay (CLIA). In the current study, the CCK-8 assay showed that ZYM promoted the proliferation of WSU-HN4, WSU-HN6 and CAL27 cells via the TLR2/MyD88 pathway. The cell adhesion assay showed that the number ofC. albicanscells per field significantly increased in ZYM-treated OSCC cells compared to controls. When treated with ZYM, OSCC cells secreted significantly more pro-inflammatory cytokine IL-1 beta, which could enhance inflammation in oral cancer microenvironment. In conclusion, ZYM from the fungal cell wall promotes the proliferation,C. albicansadhesion and IL-1 beta production in OSCC, as demonstrated by in vitro experiments.