Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture
Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture
复制标题
DOI：
10.1128/jb.182.12.3593-3596.2000
复制
发表时间：
2000-06-01
影响因子：
3.2
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
Danese, PN;Pratt, LA;Kolter, R
虽然胞外多糖（EPSs）是细菌生物膜的重要组成部分，但它们对生物膜结构和功能的贡献仅在少数生物中得到了研究。在这些情况下，EPS已被证明是细胞附着于非生物表面所必需的。在这里，我们采用遗传方法来检查大肠杆菌K-12的EPS结肠酸在生物膜形成中的潜在作用。无论是熟练或缺乏可拉酸生产菌株呻吟，并允许粘附在非生物表面，然后进行宏观和微观检查。令人惊讶的是，我们发现可拉酸的产生并不需要表面附着。相反，结肠酸对于形成复杂的三维结构和大肠杆菌生物膜的深度至关重要。
Although exopolysaccharides (EPSs) are a large component of bacterial biofilms, their contribution to biofilm structure and function has been examined for only a few organisms. In each of these cases EPS has been shown to be required for cellular attachment to abiotic surfaces. Here, we undertook a genetic approach to examine the potential role of colanic acid, an EPS of Escherichia coli K-12, in biofilm formation. Strains either proficient or deficient in colanic acid production were groan and allowed to adhere to abiotic surfaces and were then examined both macroscopically and microscopically. Surprisingly, we found that colanic acid production is not required for surface attachment. Rather, colanic acid is critical for the formation of the complex three-dimensional structure and depth of E. coli biofilms.