Effects of oxymatrine on sympathoexcitatory reflex induced by myocardial ischemic signaling mediated by P2X3 receptors in rat SCG and DRG
Effects of oxymatrine on sympathoexcitatory reflex induced by myocardial ischemic signaling mediated by P2X3 receptors in rat SCG and DRG
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DOI：
10.1016/j.brainresbull.2011.01.011
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发表时间：
2011-04-05
影响因子：
3.8
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
--
作者：
Li, Guilin;Liu, Shuangmei;Liang, Shangdong
交感兴奋反射的特点是血压升高和交感神经活动增加。心肌缺血（MI）损伤后，SCG神经元中的P2X(3)受体参与增强交感兴奋反射。本研究旨在探讨氧化苦参碱（Oxy）对大鼠颈上神经节（SCG）和颈背根神经节（DRG）交感兴奋反射中P2X(3)受体介导的心肌缺血信号传递的影响。本研究采用免疫组化、原位杂交和Western blotting方法分析SCG和DRG神经元中P2X(3)免疫反应性、mRNA和蛋白的表达水平。结果表明：心肌缺血损伤诱导：收缩压和心率升高，上调SCG和DRG神经元中P2X(3)受体的表达。SCG和DRG神经元中P2X(3)受体的上调表达随后导致交感兴奋反射加重。氧化苦参碱降低心肌缺血大鼠的收缩压和心率。心肌缺血大鼠经氧化苦参碱处理后，P2X(3)免疫反应性、mRNA和蛋白表达水平均低于心肌缺血大鼠。氧化苦参碱可降低P2X(3)受体的表达，抑制大鼠SCG和DRG神经元P2X(3)受体对心肌缺血损伤的伤害性传递所引起的交感兴奋反射加重。(C) 2011爱思唯尔公司版权所有。
Sympathoexcitatory reflex is characterized by an increase in blood pressure and sympathetic nerve activity. P2X(3) receptors in SCG neurons are involved in increasing sympathoexcitatory reflex after myocardial ischemic (MI) injury. The present study is aimed to explore the effects of oxymatrine (Oxy) on the transmission of myocardial ischemic signaling mediated by P2X(3) receptors in rat superior cervical ganglia (SCG) and cervical dorsal root ganglia (DRG) in the sympathoexcitatory reflex after myocardial ischemic injury. In this study, the expression levels of P2X(3) immunoreactivity, mRNA and protein were analyzed in SCG and DRG neurons by immunohistochemistry, in situ hybridization and Western blotting. The results show that the myocardial ischemic injury induce:; the increase of the systolic blood pressure and heart rate and upregulates the expression of P2X(3) receptors in SCG and DRG neurons. Upregulated expression of P2X(3) receptors in SCG and DRG neurons subsequently leads to the aggravated sympathoexcitatory reflex. Oxymatrine reduces the systolic blood pressure and heart rate in myocardial ischemic rats. After myocardial ischemic rats are treated with oxymatrine, the expression levels of P2X(3) immunoreactivity, mRNA and protein are lower than those in myocardial ischemic rats. Oxymatrine may decrease the expression of P2X(3) receptor and depress the aggravated sympathoexcitatory reflex induced by the nociceptive transmission of myocardial ischemic injury via P2X(3) receptors of rat SCG and DRG neurons. (C) 2011 Elsevier Inc. All rights reserved.