Inhibitory effect of chondroitin sulfate oligosaccharides on bovine testicular hyaluronidase.
Inhibitory effect of chondroitin sulfate oligosaccharides on bovine testicular hyaluronidase.
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硫酸软骨素寡糖对牛睾丸透明质酸酶的抑制作用。
DOI：
10.1016/j.carbpol.2014.11.071
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Kakizaki;I.;Koizumi;H.;Chen;F.;Endo;M.
透明质酸和硫酸软骨素是动物组织细胞外基质的重要组成部分；然而，它们的功能及其寡糖结构尚未完全阐明。以透明质酸为底物，制备了透明质酸寡糖和硫酸软骨素寡糖，研究了它们对牛睾丸透明质酸酶水解和转糖基化反应的影响。通过高效液相色谱法分析产物在独立反应体系中的水解活性和转糖基化活性。硫酸软骨素寡糖对牛睾丸透明质酸酶的水解和转糖基化反应有剂量依赖性的抑制作用，而对透明质酸和软骨素寡糖无剂量依赖性抑制作用。对透明质酸寡糖水解反应的动力学分析表明，硫酸软骨素寡糖的抑制模式是竞争性的。
Hyaluronan and chondroitin sulfates are prominent components of the extracellular matrices of animal tissues; however, their functions in relation to their oligosaccharide structures have not yet been fully elucidated. The oligosaccharides of hyaluronan and chondroitin sulfate were prepared and used to investigate their effects on the hydrolysis and transglycosylation reactions of bovine testicular hyaluronidase when hyaluronan was used as a substrate. Hydrolysis and transglycosylation activities were assessed in independent reaction systems by analyzing the products by HPLC. The hydrolysis and transglycosylation reactions of bovine testicular hyaluronidase were dose-dependently inhibited by chondroitin sulfate oligosaccharides, but not by hyaluronan or chondroitin oligosaccharides. A kinetic analysis of the hydrolysis reaction using hyaluronan octasaccharide revealed that the inhibition mode by chondroitin sulfate oligosaccharides was competitive.