Solution-Phase Electrochemical Aptamer-Based Sensors
Solution-Phase Electrochemical Aptamer-Based Sensors
复制标题
基于溶液相电化学适体的传感器
DOI：
10.1109/tbme.2022.3203026
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发表时间：
2023
影响因子：
4.6
通讯作者：
Heikenfeld, Jason
中科院分区：
文献类型：
--
作者：
Yuan, Yuchan;Bali, Ahilya;White, Ryan J.;Heikenfeld, Jason
基于适配体的电化学传感器（EABs）在金工作电极上使用自组装的单层，现已在多种分析物中进行了体内验证，即使在连续传感格式下也证明了它们的灵敏度和特异性。然而，其寿命仅为24小时，并且对于高度稀释的分析物（nM体系）的敏感性具有挑战性。本文报道了一种基于电化学适配体的传感方法，该方法不是与金加工电极共价结合，而是适配体在溶液中自由移动。这种替代方法有可能通过减少电极表面降解和使用适体结合结构提高灵敏度来提高寿命，当适体与电极共价结合时，适体是不可用的。具体来说，将分子信标（荧光）皮质醇适体应用于安培法溶液皮质醇EAB传感器，在仅10 nM处显示出~ 5%的信号增益，在几μM处显示出~ 70%的饱和信号增益。由于使用亚甲基蓝氧化还原标记的适体，通过交叉指状电极的安培法测量，获得了稳健的信号。虽然这一结果证明了液相EAB传感器的基本可行性，但该结果还需要在金工作电极上自组装单层烷基硫酸盐堵塞层，这限制了设备的潜在寿命。这些结果累积表明，溶液相EAB传感器的初始意义可能对护理点类型的测试应用最强，并且需要进一步开发持久的连续传感应用。
Electrochemical aptamer-based sensors (EABs) using self-assembled monolayers on gold working-electrodes have now been in-vivo demonstrated for multiple-analytes, demonstrating their sensitivity and specificity even in a continuous sensing format. However, longevity has been demonstrated for only 24 hours and sensitivity has been challenging for highly dilute analytes (nM regime). A novel approach is reported here using electrochemical aptamer-based sensing that is not covalently-bound to a gold-working electrode but where aptamers are freely mobile in solution. This alternative approach has the potential to improve longevity by reducing electrode surface degradation and improving sensitivity using aptamer binding constructs that are not available for aptamers when covalently bound to the electrode. Specifically, a molecular-beacon (fluorescent) cortisol aptamer was adapted into an amperometry solution-phase cortisol EAB sensor, demonstrating ∼5% signal gain starting at only 10 nM and a saturated signal gain of ∼70% at several μM. A robust signal was achieved due to use of methylene-blue redox-tagged aptamer that was measured through amperometry with interdigitated electrodes. While this result demonstrates the basic feasibility of solution-phase EAB sensors, the result also required a self-assembled monolayer alkylthiolate blocking-layer on the gold working electrode which restricts potential device longevity. These results cumulatively suggest that initial significance of solution-phase EAB sensors may be strongest for point-of-care type testing applications and further development would be required for long-lasting continuous sensing applications.
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影响因子：
4
作者：
Yang KA;Chun H;Zhang Y;Pecic S;Nakatsuka N;Andrews AM;Worgall TS;Stojanovic MN
通讯作者：
Stojanovic MN
影响因子：
3.9
作者：
DELAMARCHE, E;MICHEL, B;GERBER, C
通讯作者：
GERBER, C
叉指微带电极：计时电流分析法和稳态电流
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
B. Seddon;H. Girault;M. Eddowes
通讯作者：
M. Eddowes
影响因子：
9.5
作者：
Shaver, Alexander;Curtis, Samuel D.;Arroyo-Curras, Netzahualcoyotl
通讯作者：
Arroyo-Curras, Netzahualcoyotl
DOI：
10.1039/b921253a
发表时间：
2010-03
期刊：
The Analyst
影响因子：
--
作者：
White RJ;Rowe AA;Plaxco KW
通讯作者：
Plaxco KW