Hydrodynamic separation of cells utilizing insulator-based dielectrophoresis
Hydrodynamic separation of cells utilizing insulator-based dielectrophoresis
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DOI：
10.1007/s00542-009-0947-6
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发表时间：
2010-07-01
影响因子：
2.1
通讯作者：
Shih, Hsin-Yuan
中科院分区：
文献类型：
--
作者：
Jen, Chun-Ping;Huang, Ching-Te;Shih, Hsin-Yuan
生物细胞的操纵和区分对于许多生物医学应用至关重要。基于绝缘体的介电电泳 (iDEP) 捕获由绝缘结构组成，该绝缘结构会挤压导电溶液中的电场以产生不均匀的电场。绝缘结构的图案被设计为产生高电场区域，以将介电电泳呈阳性的细胞（例如低频下死亡的哺乳动物细胞）捕获在较低流场区域中。然而，负介电泳细胞（例如，低频下的活细胞）被排斥到速度较高的低电场区域。因此，具有不同介电泳反应的细胞被分离并收集在出口处。通过数值模拟来研究本研究中的设计参数。此外，还进行了实验来证明在所提出的设计中使用 iDEP 进行水力分离的可行性。
Manipulation and discrimination of biological cells is essential to many biomedical applications. Insulator-based dielectrophoresis (iDEP) trapping consists of insulating structures which squeeze the electric field in a conductive solution to create a non-uniform electric field. The pattern of insulating structure was designed to generate regions of high-electric field to trap cells with positive dielectrophoresis (e.g., dead mammalian cells at low frequency) in lower-flow-field regions. However, negative-dielectrophoretic cells (e.g., viable cells at low frequency) were repelled toward low-electric-field regions where the velocity was higher. Cells with different dielectrophoretic responses were therefore separated and collected in the outlet. Simulations were numerically performed to investigate parameters of the design in the present study. Furthermore, experiments were also conducted to demonstrate the feasibility of hydrodynamic separation using iDEP in the proposed design.