Gene expression profiling of changes induced by maternal diabetes in the embryonic heart
Gene expression profiling of changes induced by maternal diabetes in the embryonic heart
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DOI：
10.1016/j.reprotox.2015.06.045
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发表时间：
2015-11-01
影响因子：
3.3
通讯作者：
Pavlinkova, Gabriela
中科院分区：
文献类型：
--
作者：
Bohuslavova, Romana;Skvorova, Lada;Pavlinkova, Gabriela
心血管缺陷是与母体糖尿病相关的最常见的先天性缺陷之一。基于全胚胎基因表达芯片分析，选择11个基因用于糖尿病暴露心脏的时间表达分析。与我们的对照组相比，在E13.5，E14.5和E18.5，大多数选定的基因在糖尿病暴露的心脏中失调。我们发现，在E10.5时，糖尿病暴露的心脏中缺氧和HIF-1 α蛋白水平增加，这是诱导与糖尿病胚胎病相关的发育缺陷的关键时间点。此外，我们发现心脏Vegfa水平升高可能引发与糖尿病胚胎病相关的发育异常。我们的研究结果表明，母体糖尿病影响参与心脏发育和组织重塑的基因编码分子的时间表达模式，这些分子可能会影响心脏成熟过程，从而影响糖尿病妊娠的最终结局。(C)2015爱思唯尔公司All rights reserved.
Cardiovascular defects are one of the most common congenital defects associated with maternal diabetes. Based on whole embryo gene expression microarray analysis, 11 genes were chosen for temporal expression analysis of diabetes-exposed hearts. The majority of the selected genes were deregulated in diabetes-exposed hearts compared to our controls at E13.5, E14.5, and E18.5. We showed increased hypoxia and HIF-1 alpha protein levels in diabetes-exposed hearts at E10.5, which is a critical time point for the induction of developmental defects associated with diabetic embryopathy. Additionally, we found increased cardiac Vegfa levels that might trigger developmental abnormalities associated with diabetic embryopathy. Our results show that maternal diabetes affects the temporal expression pattern of gene encoding molecules involved in heart development and tissue remodelling and that these molecules might affect heart maturation processes and thus, the final outcome of diabetic pregnancies. (C) 2015 Elsevier Inc. All rights reserved.