Fermentative production of 1-propanol from D-glucose, L-rhamnose and glycerol using recombinant Escherichia coli
Fermentative production of 1-propanol from D-glucose, L-rhamnose and glycerol using recombinant Escherichia coli
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DOI：
10.1016/j.jbiosc.2016.03.011
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Kataoka, Michihiko
中科院分区：
文献类型：
--
作者：
Matsubara, Mitsuru;Urano, Nobuyuki;Kataoka, Michihiko
研究了利用代谢工程大肠杆菌从D-葡萄糖、L-鼠李糖和甘油发酵生产1-丙醇的方法。目的：研究重组大肠杆菌转化1，2-丙二醇（1，2-PD）生产1-丙醇的能力.克隆了肺炎克雷伯氏菌（Klebsiella pneumoniae）的二醇脱氢酶基因、丙醇脱氢酶基因和腺苷钴胺素（adenosylcobalamin，ACBl）再生酶基因等pdu调节子的一部分，构建了表达载体pRSF_pduCDEGHOQS。重组大肠在我们先前的报道中构建了含有1，2-PD合成途径（pICK_mde）基因的pRSF_pduCDEGHOQS的大肠杆菌（Urano等，应用微生物学生物技术，99，2001-2008，2015），在培养72小时后以036 mol/mol的摩尔产率从D-葡萄糖产生16. ImM的1-丙醇。29.9使用E. con仅携带pRSF_pduCDEGHOQS。此外，通过将ATP依赖的二羟丙酮激酶基因加入到E. pICK_mde和pRSF_pduCDEGOQS的表达载体。在所有情况下，1-丙醇的生产都是通过仅加入少量的C2 H2 Cl 2来实现的。(C)2016年，日本生物技术学会。All rights reserved .
Fermentative production of 1-propanol, which is one of the promising precursors of polypropylene production, from D-glucose, L-rhamnose and glycerol using metabolically engineered Escherichia coli was examined. To confer the ability to produce 1-propanol from 1,2-propanediol (1,2-PD) in recombinant E. coli, a part of the pdu regulon including the diol dehydratase and the propanol dehydrogenase genes together with the adenosylcobalamin (AdoCbl) regeneration enzyme genes of Klebsiella pneumoniae was cloned, and an expression vector for these genes (pRSF_pduCDEGHOQS) was constructed. Recombinant E. coli harboring pRSF_pduCDEGHOQS with 1,2-PD synthetic pathway (pICK_mde) genes, which was constructed in our previous report (Urano et al., Appl. Microbiol. Biotechnol., 99, 2001-2008, 2015), produced 16.1 mM of 1-propanol from D-glucose with a molar yield of 036 mol/mol after 72 h cultivation. 29.9 mM of 1-propanol was formed from L-rhamnose with a molar yield of 0.81 mol/mol using E. con carrying only pRSF_pduCDEGHOQS. In addition, 1-propanol production from glycerol was achieved by addition of the ATP-dependent dihydroxyacetone kinase gene to E. coli harboring pICK_mde and pRSF_pduCDEGOQS. In all cases, 1-propanol production was achieved by adding only a small amount of AdoCbl. (C) 2016, The Society for Biotechnology, Japan. All rights reserved .