Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles
Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles
复制标题
尿素增强金纳米颗粒表面等离子体共振光散射一步法测定人尿卡那霉素
DOI：
10.1007/s00216-009-3134-9
复制
发表时间：
2009-12-01
影响因子：
4.3
通讯作者：
Li, Na
中科院分区：
文献类型：
--
作者：
Wang, Xinyi;Zou, Mingjian;Li, Na
本研究旨在建立一种简便、灵敏的人尿中卡那霉素（KANA）的分析方法。通过KANA增强金纳米颗粒（AuNPs）的等离子体共振光散射（PRLS）为该分析方法提供了基础。在pH 6.7时，KANA诱导具有增强的PRLS的AuNPs聚集。通过添加尿素进一步增强了AuNPs-KANA系统的PRLS。KANA的线性范围和检测限分别为20-800 nmol L-1和2 nmol L-1。尿液中存在的潜在干扰物质对测定的影响可忽略不计，因此无需进行初步样品分离。加标人尿液中KANA的回收率为94- 104%。这种简单、灵敏的方法，利用尿素增强AuNPs-KANA体系的PRLS，可能为测定富含OH基团的化合物提供一种新的方法。图KANA诱导AuNPs聚集和尿素增强的示意图，包括放大说明KANA和尿素之间的N···H-O氢键
The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs–KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20–800 nmol L−1and 2 nmol L−1, respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94–104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs–KANA system, may provide a new approach for determination of compounds rich in OH groups.FigureSchematic diagram of KANA-induced aggregation of AuNPs and enhancement by urea, including magnification for illustration of N···H-O hydrogen bond between KANA and urea