Paludibacter propionicigenes gen. nov., sp nov., a novel strictly anaerobic, Gram-negative, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil in Japan
Paludibacter propionicigenes gen. nov., sp nov., a novel strictly anaerobic, Gram-negative, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil in Japan
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DOI：
10.1099/ijs.0.63896-0
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Ueki, K
中科院分区：
文献类型：
--
作者：
Ueki, A;Akasaka, H;Ueki, K
从日本缺氧稻田土壤中分离到一株严格厌氧的丙酸产生菌(WB4(T))，对其表型和系统发育进行了研究。细胞革兰氏阴性，不活动，不形成孢子，短杆。该菌株利用多种糖类，以丙酸和乙酸酯为主要发酵产物，并含有少量琥珀酸。最适生长温度为30℃，氧化酶、过氧化氢酶和硝酸盐还原活性均为阴性。细胞内主要脂肪酸为Anteiso-C-15：0、C-15：0和Anteiso-C-17：03-OH。其中最主要的呼吸性苯二酚是菜籽酮MK-8(H-4)。基因组DNA G+C含量为39.3mol%。基于16S rRNA基因序列的系统发育分析将该菌株归入类杆菌门。与菌株WB4(T)的亲缘关系最近的是一个来自被马粪污染的水中的环境克隆(序列相似性为99.7%)，该菌株与主要来自淡水沉积物的其他环境克隆形成了明显的聚类。与之最接近的是Dysgonomonas属，16S rRNA基因序列相似性为90.9%~89.8%。其次是拟杆菌属(Bacteroids Merdae)，与模式菌株的相似性为88.7%。鉴于菌株WB4(T)的生态、生理和化学分类特征不同于任何近缘种，故将其命名为一新属新种--丙酸帕鲁迪杆菌属。Nov.，sp.11月，被提议为适应这一点。模式菌株为WB4(T)(=JCM 13257(T)=DSM 17365(T))。
A strictly anaerobic, propionate-producing bacterial strain (WB4(T)) isolated from rice plant residue in anoxic rice-field soil in Japan was characterized phenotypically and phylogenetically. Cells were Gram-negative, non-motile, non-spore-forming, short rods. The strain utilized various sugars and produced propionate and acetate as major fermentation products with a small amount of succinate. The optimum growth temperature was 30 degrees C. Oxidase, catalase and nitrate-reducing activities were negative. The major cellular fatty acids were anteiso-C-15:0, C-15:0 and anteiso-C-17:0 3-OH. Menaquinone MK-8(H-4) was the major respiratory quinone. The genomic DNA G + C content was 39.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence placed the strain in the phylum 'Bacteroidetes'. The closest relative to strain WB4(T) was an environmental clone from water contaminated with equine manure (sequence similarity of 99.7%) and the strain formed a distinct cluster with other environmental clones mainly from freshwater sediments. The closest recognized species were members of the genus Dysgonomonas, with 1 6S rRNA gene sequence similarities of 90.9-89.8%. Bacteroides merdae was the next closest recognized species (similarity of 88.7% to the type strain). Given that the ecological, physiological and chemotaxonomic characteristics of strain WB4(T) were different from those of any related species, a new genus and species Paludibacter propionicigenes gen. nov., sp. nov., is proposed to accommodate it. The type strain is WB4(T) (=JCM 13257(T) =DSM 17365(T)).