On the unreliability of published DNA sequences
On the unreliability of published DNA sequences
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DOI：
10.1046/j.1469-8137.2003.00861.x
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发表时间：
2003-10-01
期刊：
影响因子：
9.4
通讯作者：
Panchal, G
中科院分区：
文献类型：
--
作者：
Bridge, PD;Roberts, PJ;Panchal, G
在这里，通过对从公共访问数据库中获得的206个命名序列进行严格重新评估来测试已发表的真菌核酸序列的可靠性。检查了核糖体RNA（rRNA）基因簇的序列，因为这些序列通常用于建立真菌系统发育和进化，并且越来越多地用于从基于非培养的研究中鉴定真菌。51个rRNA内转录间隔区（ITS）鹅膏菌属的55个ITS序列、茎点霉属的55个ITS序列和阳菌目代表属的100个rRNA小亚基序列。在每种情况下，真菌组的选择部分基于三个或更多个实验室保存的序列，以避免样品偏差。结果表明，每个群体中多达20%的序列可能是不可靠的，这一比例得到了其他非正式观察的支持。
Here, the reliability of published fungal nucleic acid sequences is tested by the critical re-evaluation of 206 named sequences obtained from public-access databases.Sequences from the ribosomal RNA (rRNA) gene cluster were examined as these are commonly used to establish fungal phylogeny and evolution, and are also increasingly employed in the identification of fungi from nonculture based studies.Fifty-one rRNA internal transcribed spacer (ITS) sequences were obtained for species of Amanita, 55 ITS sequences were obtained for species of Phoma and 100 rRNA small subunit sequences were obtained from representative genera of the order Helotiales. in each case, the fungal group was selected partly on the basis of sequences deposited by three or more laboratories in order to avoid sample bias. The results suggest that up to 20% of the sequences available for each group may be unreliable, and this proportion is supported by additional informal observations.