Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination
Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination
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DOI：
10.1073/pnas.0604205103
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发表时间：
2006-09-12
影响因子：
11.1
通讯作者：
Hirabayashi, Masumi
中科院分区：
文献类型：
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作者：
Shinohara, Takashi;Kato, Megumi;Hirabayashi, Masumi
精原细胞移植为研究精子发生提供了一个独特的机会，并为精原干细胞的检测提供了一种方法。然而，在异种环境中成熟的生殖细胞功能是否正常仍然是未知的。在这项研究中，我们证明了成功的生产异种后代，通过使用精原细胞移植。从未成熟睾丸中分离大鼠精原干细胞，将其移植到白消安处理的裸鼠睾丸曲细精管中。利用大鼠精子细胞或异种代孕小鼠中发育的精子，将新鲜和冷冻保存的供体细胞与大鼠卵母细胞显微授精后，产生大鼠后代。这些后代是可生育的，并且具有正常的印记模式。利用种间生殖细胞移植和体外显微授精技术生产异种后代将成为动物转基因和物种保护的有力工具。
Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.