MODIFICATION OF CELL LETHALITY AT ELEVATED-TEMPERATURES - PH EFFECT
MODIFICATION OF CELL LETHALITY AT ELEVATED-TEMPERATURES - PH EFFECT
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DOI：
10.2307/3574745
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发表时间：
1977-01-01
影响因子：
3.4
通讯作者：
GERWECK, LE
中科院分区：
文献类型：
--
作者：
GERWECK, LE
在选定的细胞外pH下测定中国仓鼠卵巢细胞对高温的致死反应。在41 - 44 ℃的温度范围内，pH从7.6降低到6.7增加了细胞的致死反应。C.在37 ℃的pH范围内，细胞活力不受影响。C.在正常pH值（7.4）下几乎致死的温度下，pH值致敏作用最为显著。暴露在42度高温下4小时。C在pH 7.4时使存活率降低至10%，在pH 6.7时降低至0.01%。当细胞同时暴露于降低的pH和升高的温度时，观察到增强的细胞杀伤。延长高温前后pH暴露时间并不影响存活率。高密度培养增加了细胞对高温的敏感性。这种影响是由于介质的代谢酸化，并可以通过调节pH值来逆转。热疗可用于治疗癌症的程度，即正常组织和肿瘤组织之间发生差异反应。
The lethal response of Chinese hamster ovary cells to hyperthermia was determined at selected extracellular pH. Decreasing pH from 7.6 to 6.7 increased the lethal response of cells over the temperature range of 41-44.degree. C. Cell viability was not affected over this pH range at 37.degree. C. The pH sensitizing affect was most prominent at temperatures which were marginally lethal at normal pH (7.4). Four hours of exposure to 42.degree. C decreased survival to 10% at pH 7.4 and 0.01% at pH 6.7. Enhanced cell killing was observed when the cells were exposed to reduced pH and elevated temperatures simultaneously. Prolonging the time of pH exposure before and after hyperthermia did not influence survival. High-density culturing increased the sensitivity of cells to hyperthermia. This affect was due to metabolic acidification of the medium and could be reversed by adjusting the pH. Hyperthermia is useful for the treatment of cancer to the extent that differential response occurs between normal and tumor tissue.