Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals
Latent Acyclovir-Resistant Herpes Simplex Virus Type 1 in Trigeminal Ganglia of Immunocompetent Individuals
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DOI：
10.1093/infdis/jis237
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发表时间：
2012-05-15
影响因子：
6.4
通讯作者：
Verjans, Georges M. G. M.
中科院分区：
文献类型：
--
作者：
van Velzen, Monique;van Loenen, Freek B.;Verjans, Georges M. G. M.
高变单纯疱疹病毒（HSV）基因胸苷激酶（TK）基因内的特定突变导致阿昔洛韦（ACV）耐药。为了揭示潜伏的ACV耐药（ACV（R））HSV-1的存在，我们确定了5名免疫活性个体的成对三叉神经节（TG）中HSV-1 TK基因库的遗传和功能变异性。潜伏病毒库由供体特异性HSV-1准种组成，包括一种主要的ACV敏感性（ACV（S））和多种遗传相关的次要ACV（S）和ACV（R）TK变体。与次要变异相反，主要TK变异在配对TG之间共享。数据表明，在人TG中存在遗传学相关的ACV（S）和ACV（R）潜伏HSV-1。
Specific mutations within the hypervariable herpes simplex virus (HSV) gene thymidine kinase (TK) gene lead to acyclovir (ACV) resistance. To uncover the existence of latent ACV-resistant (ACV(R)) HSV-1, we determined the genetic and functional variability of the HSV-1 TK gene pool in paired trigeminal ganglia (TG) of 5 immunocompetent individuals. The latent virus pool consisted of a donor-specific HSV-1 quasispecies, including one major ACV-sensitive (ACV(S)) and multiple phylogenetic-related minor ACV(S) and ACV(R) TK variants. Contrary to minor variants, major TK variants were shared between paired TG. The data demonstrate the coexistence of phylogenetic-related ACV(S) and ACV(R) latent HSV-1 in human TG.