Production of flavonol and flavone 6-C-glucosides by bioconversion in Escherichia coli expressing a C-glucosyltransferase from wasabi (Eutrema japonicum)
Production of flavonol and flavone 6-C-glucosides by bioconversion in Escherichia coli expressing a C-glucosyltransferase from wasabi (Eutrema japonicum)
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DOI：
10.1007/s10529-021-03165-3
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发表时间：
2021-07-24
影响因子：
2.7
通讯作者：
Taguchi, Goro
中科院分区：
文献类型：
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作者：
Dorjjugder, Nasanjargal;Hatano, Mayu;Taguchi, Goro
目的 使用表达芥末 C-葡萄糖基转移酶 (WjGT1) 的重组大肠杆菌进行生物转化，生产黄酮醇和黄酮 6-C-葡萄糖苷。结果表达WjGT1（Ec-WjGT1）的大肠杆菌在补充有葡萄糖的M9基本培养基中将黄酮（芹菜素和木犀草素）和黄酮醇（槲皮素和山奈酚）转化为6-C-葡萄糖苷，并将这些产物释放到培养基中。 Ec-WjGT1 系统还可将黄烷酮（柚皮素）转化为其 C-葡萄糖苷，6 小时内转化率为 60%。为了扩大生产，每15-60分钟将芹菜素、山奈酚和槲皮素以20-50μM的浓度依次加入Ec-WjGT1系统中，然后该系统能够以89-99%的转化率生产异牡荆素、山奈酚6-C-葡萄糖苷和槲皮素6-C-葡萄糖苷。结论 当采用顺序给药以避免底物沉淀时，Ec-WjGT1 系统可以快速、轻松地以高转化率产生黄酮和黄酮醇 6-C-葡萄糖苷。
Objectives To produce flavonol and flavone 6-C-glucosides by bioconversion using recombinant Escherichia coli expressing a C-glucosyltransferase from wasabi (WjGT1). Results Escherichia coli expressing WjGT1 (Ec-WjGT1) converted flavones (apigenin and luteolin) and flavonols (quercetin and kaempferol) into their 6-C-glucosides in M9 minimal media supplemented with glucose, and released these products into the culture media. Ec-WjGT1 system also converts a flavanone (naringenin) into its C-glucoside at a conversion rate of 60% in 6 h. For scale-up production, apigenin, kaempferol, and quercetin were sequentially fed into the Ec-WjGT1 system at concentrations of 20-50 mu M every 15-60 min, and the system was then able to produce isovitexin, kaempferol 6-C-glucoside, and quercetin 6-C-glucoside at an 89-99% conversion rate. Conclusions The Ec-WjGT1 system quickly and easily produces flavone and flavonol 6-C-glucosides at high conversion rates when using sequential administration to avoid precipitation of substrates.