Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH17 cytokine interleukin-22
Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH17 cytokine interleukin-22
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DOI：
10.1182/blood-2008-12-192443
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发表时间：
2009-04-23
期刊：
影响因子：
20.3
通讯作者：
Caligiuri, Michael A.
中科院分区：
文献类型：
--
作者：
Hughes, Tiffany;Becknell, Brian;Caligiuri, Michael A.
通过对不同的NK细胞亚群的表征，揭示了人类自然杀伤(NK)细胞中相当大的功能异质性。因此，CD56(+)NKp44(+)NK细胞的一小部分，被称为NK-22细胞，最近在次级淋巴组织(SLT)中被描述为静息时的IL-22(-)，该群体中的一小部分在激活时成为IL-22(-)。我们发现，SLT中绝大多数第3阶段的未成熟NK(INK)细胞结构性和选择性地表达IL-22，这是一种对粘膜免疫至关重要的T(H)17细胞因子，而NK发育早期和后期的中间产物不表达IL-22。这些墨水细胞的表面表型为CD34(-)、CD117(+)、CD161(+)、CD94(-)，大部分缺乏NKp44和CD56的表达，不产生干扰素-γ，也不具有细胞杀伤活性。综上所述，第三阶段墨汁细胞高度富含IL-22和IL-26信使RNA，并产生IL-22蛋白，但不表达IL-17A或IL-17F。(血。2009；113：4008-4010)
Considerable functional heterogeneity within human natural killer (NK) cells has been revealed through the characterization of distinct NK-cell subsets. Accordingly, a small subset of CD56(+)NKp44(+)NK cells, termed NK-22 cells, was recently described within secondary lymphoid tissue (SLT) as IL-22(-) when resting, with a minor fraction of this population becoming IL-22(-) when activated. Here we discover that the vast majority of stage 3 immature NK (iNK) cells in SLT constitutively and selectively express IL-22, a T(H)17 cytokine important for mucosal immunity, whereas earlier and later stages of NK developmental intermediates do not express IL-22. These iNK cells have a surface phenotype of CD34(-)CD117(+)CD161(+)CD94(-), largely lack expression of NKp44 and CD56, and do not produce IFN-gamma or possess cytolytic activity. In summary, stage 3 iNK cells are highly enriched for IL-22 and IL-26 messenger RNA, and IL-22 protein production, but do not express IL-17A or IL-17F. (Blood. 2009;113:4008-4010)