Toxicological and biochemical basis of synergism between the entomopathogenic fungus Lecanicillium muscarium and the insecticide matrine against Bemisia tabaci (Gennadius).
Toxicological and biochemical basis of synergism between the entomopathogenic fungus Lecanicillium muscarium and the insecticide matrine against Bemisia tabaci (Gennadius).
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DOI：
10.1038/srep46558
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发表时间：
2017-04-20
影响因子：
4.6
通讯作者：
Qiu BL
中科院分区：
文献类型：
--
作者：
Ali S;Zhang C;Wang Z;Wang XM;Wu JH;Cuthbertson AGS;Shao Z;Qiu BL
用不同浓度的苦参碱(杀虫剂)和粉锈菌(昆虫病原真菌)的不同组合对甘薯烟粉虱进行了为害试验。本研究结果表明，苦参碱和毒蛾乳杆菌对烟粉虱死亡率和酶活性有协同作用。为了阐明烟粉虱对这两种控制剂的解毒和免疫反应的生化机制，对不同解毒和抗氧化酶的活性进行了定量研究。苦参碱和毒蛾联合使用后，羧酸链酶(CARE)、谷胱甘肽-S-转移酶(GSTS)和几丁质酶(CHI)活性在感染初期下降。在整个实验期间，乙酰胆碱酯酶(AChE)活性升高，而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性在感染后期下降。苦参碱和烟粉虱对烟粉虱的致死率增加和对酶反应的抑制表明两种药剂之间有很强的协同作用。这种强烈的协同作用可能与烟粉虱作为靶标昆虫乙酰胆碱(Ach)受体的苦参碱和毒蛾的乙酰胆碱(Ach)受体的乙酰胆碱平衡紊乱和AChE活性改变有关。因此，我们的结果揭示了苦参碱和毒蛾对烟粉虱的协同作用所涉及的复杂的生化过程。
The sweetpotato whitefly Bemisia tabaci (Gennadius) was challenged with different combinations of matrine (insecticide) and Lecanicillium muscarium (entomopathogenic fungus). Our results revealed a synergistic relationship between matrine and L. muscarium on mortality and enzyme activities of B. tabaci. To illustrate the biochemical mechanisms involved in detoxification and immune responses of B. tabaci against both control agents, activities of different detoxifying and antioxidant enzymes were quantified. After combined application of matrine and L. muscarium, activities of carboxylestrease (CarE), glutathione-s-transferase (GSTs) and chitinase (CHI) decreased during the initial infection period. Acetylcholinestrase (AChE) activities increased during the entire experimental period, whereas those of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) decreased during the later infection period. The increased mortality and suppression of enzymatic response of B. tabaci following matrine and L. muscarium application suggests a strong synergistic effect between both agents. The strong synergistic effect is possibly related to the disturbance of acetylcholine balance and changes in AchE activities of the whitefly as both matrine and L. muscarium target insect acetylcholine (Ach) receptors which in turn effects AchE production. Therefore, our results have revealed the complex biochemical processes involved in the synergistic action of matrine and L. muscarium against B. tabaci.