A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
复制标题
基于CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器检测载脂蛋白E4
DOI：
10.5246/jcps.2021.03.019
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发表时间：
2021-03
影响因子：
--
通讯作者：
Ling Xiaomei
中科院分区：
文献类型：
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作者：
Zhang Ying;Li Cong;Liu Yi;Chu Lin;Zou Juncheng;Yan Youqi;Hu Xin;Ling Xiaomei
载脂蛋白E4（APOE 4）是晚发性阿尔茨海默病（AD）的重要危险因子，但目前APOE 4的检测方法进展甚微，且大多数检测方法耗时长、仪器昂贵。该免疫传感器在10 ~ 100 ng/mL范围内线性关系良好，检出限为1.8 ng/mL（S/N=3），具有良好的选择性、重现性和稳定性，可用于人血清样品中APOE 4的灵敏测定，为APOE 4的检测和AD的早期临床预防提供了有力的支持。
Human apolipoprotein E4(APOE4)is an important risk factor for late-onset Alzheimer's disease(AD).However,little progress has been made for the detection of APOE4,and most of existing detection methods suffer from time-consuming process and expensive instruments.This study firstly proposed a simple and sensitive electrochemical method for detection of APOE4 based on carboxyl-rich CeZnO nanoparticles.Under the optimal conditions,the fabricated immunosensor exhibited a good linear relationship ranging from 10 to 100 ng/mL with the detection limit of 1.8 ng/mL(S/N=3).The proposed electrochemical immunosensor had excellent selectivity,reproducibility and stability.Good performance was observed for sensitive determination of APOE4 in human serum sample,which provided a strong support for the detection of APOE4 and early clinical prevention of AD.