Technical note: Vaginal absorption of 1,25(OH)2D3 in cattle.
Technical note: Vaginal absorption of 1,25(OH)2D3 in cattle.
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技术说明：牛的阴道吸收 1,25(OH)2D3。
DOI：
10.3168/jds.s0022-0302(04)73364-0
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发表时间：
2004
影响因子：
3.5
通讯作者：
M. Koiwa
中科院分区：
文献类型：
--
作者：
N. Okura;N. Yamagishi;Y. Naito;K. Kanno;M. Koiwa
尽管已经测试了通过静脉内、肌内和口服途径给予外源性1，25-二羟基维生素D3 [1，25（OH）2D 3]在预防奶牛分娩性低钙血症中的有效性，但是关于阴道内给药的报道很少。在本研究中，通过牛阴道腔给予1，25（OH）2D 3，并测量血液化学的后续变化，包括1，25（OH）2D 3和几种矿物质的水平，以确认阴道吸收。5头小母牛中的每头接受1 μ g 1，25（OH）2D 3/kg体重的单次阴道内给药;一头对照小母牛仅接受乙醇载体。在接受1，25（OH）2D 3的小母牛中，血浆1，25（OH）2D 3水平在2小时内较基线（88.3 +/- 20.3 pg/mL）显著升高，并在给药后6小时达到峰值（1967.4 +/- 1139.6 pg/mL）。血浆Ca水平从基线（10.4 +/- 0.4 mg/dL）升高至24 h时的峰值11.96 +/- 0.7 mg/dL。血浆中无机磷酸盐水平随时间推移从7.3 +/- 0.5 mg/dL增加至6 h时的8.1 +/- 0.8 mg/dL，并在治疗后24至96 h维持在平台水平（9.1 +/- 0.7至8.6 +/- 0.6 mg/dL）。血浆镁从基线水平2.1 +/- 0.1 mg/dL降至平台水平1.8 +/- 0.1 mg/dL，并在治疗后24 - 48 h持续。本研究提供了从牛阴道壁吸收外源性1，25（OH）2D 3的证据，如给药后2 h血浆1，25（OH）2D 3水平显著升高所示，并表明阴道内给药1，25（OH）2D 3预防低钙血症的可能效用。
Although exogenous 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] administered via intravenous, intramuscular, and oral routes has been tested for efficacy in preventing parturient hypocalcemia in dairy cows, there are few reports concerning intravaginal administration. For this study, 1,25(OH)2D3 was administered via the bovine vaginal lumen, and subsequent changes in blood chemistry, including levels of 1,25(OH)2D3 and several minerals, were measured to confirm vaginal absorption. Each of 5 heifers received a single intravaginal dose of 1 microg of 1,25(OH)2D3/kg body weight; a single control heifer received the ethanol carrier alone. In heifers receiving 1,25(OH)2D3, the plasma 1,25(OH)2D3 levels increased markedly from baseline (88.3 +/- 20.3 pg/mL) within 2 h and reached a peak at 6 h after treatment (1967.4 +/- 1139.6 pg/mL). Plasma Ca levels increased from baseline (10.4 +/- 0.4 mg/dL) to a peak of 11.96 +/- 0.7 mg/dL at 24 h. The levels of inorganic phosphate in plasma increased over time from 7.3 +/- 0.5 to 8.1 +/- 0.8 mg/dL by 6 h and were maintained at a plateau level (9.1 +/- 0.7 to 8.6 +/- 0.6 mg/dL) from 24 to 96 h after treatment. Plasma magnesium decreased from a baseline level of 2.1 +/- 0.1 mg/dL to a plateau level of 1.8 +/- 0.1 mg/dL, which was sustained from 24 to 48 h after treatment. The present study provides evidence of the absorption of exogenous 1,25(OH)2D3 from the bovine vaginal wall, as shown by the marked elevation of plasma 1,25(OH)2D3 levels by 2 h after administration, and indicates the possible utility of intravaginal administration of 1,25(OH)2D3 for prophylaxis of hypocalcemia.
维生素 D 和膳食镁对镁代谢的影响。
DOI：
10.1152/ajpendo.1980.239.6.e515
发表时间：
1980
期刊：
The American journal of physiology
影响因子：
--
作者：
Levine,BS;Brautbar,N;Walling,MW;Lee,DB;Coburn,JW
通讯作者：
Coburn,JW