Centromeric heterochromatin in the cattle rob(1;29) translocation:: α-satellite I sequences, in-situ MspI digestion patterns, chromomycin staining and C-bands
Centromeric heterochromatin in the cattle rob(1;29) translocation:: α-satellite I sequences, in-situ MspI digestion patterns, chromomycin staining and C-bands
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DOI：
10.1023/a:1009290125305
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Guedes-Pinto, H
中科院分区：
文献类型：
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作者：
Chaves, R;Heslop-Harrison, JS;Guedes-Pinto, H
研究了携带易位的葡萄牙牛 Barrosa 的 1、29 号染色体和 rob(1;29) 易位的着丝粒区域和 α-卫星 I 序列。 Rob(1;29) 着丝粒区域用碘化丙啶显示出异染色带，但与近端着丝粒常染色体不同，用染色霉素 A3 没有显示出强的着丝粒带。尽管在所有近端着丝粒染色体（包括 1 和 29）的着丝粒上都存在 α-卫星 I 序列，但在该品种的 X、Y 和 rob(1;29) 染色体的着丝粒上未发现 α-卫星 I 序列。MspI 的限制性内切酶显带揭示了着丝粒和居间区域中不同 rob(1;29) 染色体之间的多态性。数据显示 rob(1;29) 染色体的着丝粒区域丢失了 α-卫星 I 序列，同时保留了其他异染色质，并表明这种重要且广泛的易位已经发生过多次。
The centromeric regions and alpha -satellite I sequence were studied on chromosomes 1, 29 and the rob(1;29) translocation in a Portuguese breed of cattle, Barrosa, carrying the translocation. Rob(1;29) centromeric regions showed heterochromatic bands with propidium iodide but, unlike the acrocentric autosomes, no strong centromeric bands were revealed with chromomycin A3. An alpha -satellite I sequence was not found at the centromeres of the X, Y and rob(1;29) chromosomes in the breed, although it was present at the centromeres of all acrocentric chromosomes including 1 and 29. Restriction enzyme banding with MspI revealed polymorphisms between different rob(1;29) chromosomes in both centromeric and intercalary regions. The data show that the centromeric region of the rob(1;29) chromosome has lost the alpha -satellite I sequences, while retaining other heterochromatin, and suggest that this important and widespread translocation has occurred multiple times.