The Tumorgenicity ofGlioblastoma Cell Line U87MG Decreased During Serial InVitro Passage
The Tumorgenicity ofGlioblastoma Cell Line U87MG Decreased During Serial InVitro Passage
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胶质母细胞瘤细胞系U87MG在体外连续传代过程中致瘤性降低
DOI：
10.1007/s10571-018-0592-7
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发表时间：
2018
影响因子：
4
通讯作者：
Songtao Qi
中科院分区：
文献类型：
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作者：
Yu Zeng;Xizhao Wang;Jizhou Wang;Renhui Yi;Hao Long;Mingfeng Zhou;Qisheng Luo;Zhihao Zhai;Ye Song;Songtao Qi
已建立的癌细胞系通常用于研究癌症。一些因素如连续传代可能会影响癌细胞系实验的重复性，但很少有研究关注这些变化。在本研究中，观察到不同的形态和降低致瘤性在晚代U87 MG细胞。体外实验进一步显示，晚代U87 MG细胞的侵袭能力低于早代，而早代和晚代U87 MG细胞的增殖和迁移能力无明显差异。特别是，我们证实了晚代U87 MG细胞表现出更多的上皮表型，在蛋白水平上PI 3 K/Akt通路和TGF-β通路表达减少。综上所述，我们的研究结果主要集中在U87 MG细胞体外连续传代过程中的变化，提示肿瘤细胞株在体外连续传代过程中，肿瘤细胞的生物学特性发生了显著变化，并出现了多个分子转变，提示有必要缩短实验跨度，用相同或相似传代的肿瘤细胞株完成实验。
Established cancer cell lines are routinely used to study cancer. Several factors such as serial passage may affect the reproducibility of experiments with cancer cell lines, but few researches focused on these changes. In the present study, different morphology and decreased tumorigenicity were observed in late passage U87MG cells. Invitro experiments further revealed that late passage U87MG cells possessed lower invasion properties than early passage, whereas no significant differences of proliferation and migration were found between early and late passage U87MG cells. In particular, we confirmed that late passage U87MG cells exhibited more epithelial phenotype with decreased PI3K/Akt pathway and TGF-β pathway expressions at protein level. In summary, our results focused on the changes of U87MG cells during serial invitro passage, suggested that passage-induced changes may lead to notable changes of biological characteristics and several molecular transitions in cancer cell lines, indicating the necessity to shorten experiment-span and accomplish experiments with the same or similar passage cancer cell strains.