EVALUATION OF THE HUMAN IMMUNE-RESPONSE TO OUTER-MEMBRANE PROTEINS OF VIBRIO-CHOLERAE
EVALUATION OF THE HUMAN IMMUNE-RESPONSE TO OUTER-MEMBRANE PROTEINS OF VIBRIO-CHOLERAE
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DOI：
10.1128/iai.44.2.439-444.1984
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
LEVINE, MM
中科院分区：
文献类型：
--
作者：
SEARS, SD;RICHARDSON, K;LEVINE, MM
以部分纯化的O1霍乱弧菌外膜制剂(OMP)为抗原，用微量酶联免疫吸附试验抗体检测系统测定了114名腹泻志愿者对4株O1霍乱弧菌实验性攻击后的免疫应答。对29例非霍乱腹泻患者(阴性对照人群)的配对血清的分析表明，净光密度&0.10的增加是显著的。在79名El Tor生物型霍乱志愿者和35名古典生物型霍乱志愿者中，分别有50%和54%的人有明显的抗OMP抗体升高。当检测同源OMP时，表现出显著升高的配对血清在同时检测血清型异源OMP时都表现出显着升高。免疫印迹技术表明，抗体反应的抗原主要是蛋白质，而不是脂多糖。脂多糖的吸收使光密度降低了12%(0-30%)，证实了抗体主要针对OMP而不是脂多糖。因此，在临床霍乱感染期间，人类对OMP有一种免疫球蛋白反应；这种反应在生物型和血清型中是恒定的。
The immune response of 114 volunteers with diarrhea after experimental challenge with 4 strains of V. cholerae O1 was characterized in a microtiter enzyme-linked immunosorbent assay antibody detection system by using a partially purified outer membrane preparation (OMP) from these strains as an antigen. Analysis of paired sera from 29 persons with noncholera diarrhea (negative control population), demonstrated that a rise in net optical density > 0.10 was significant. Of the 79 cholera volunteers challenged with El Tor biotype and the 35 volunteers challenged with classical biotype, 50 and 54%, respectively, had significant rises in IgG anti-OMP. Paired sera that showed significant rises when tested against homologous OMP all manifested significant rises when also tested against a serotype-heterologous OMP. Immunoblotting techniques showed that the antigens to which antibodies were reacting were mainly protein in nature, not lipopolysaccharide. Absorption with lipopolysaccharide decreased the optical density by a mean of only 12% (0-30%), corroborating that antibody was mainly directed against OMP and not lipopolysaccharide. Thus, there is a human IgG response to OMP during clinical cholera infection; this response is constant among bio- and serotypes.