Effect of 40-kHz ultrasound on acute thrombotic ischemia in a rabbit femoral artery thrombosis model - Enhancement of thrombolysis and improvement in capillary muscle perfusion
Effect of 40-kHz ultrasound on acute thrombotic ischemia in a rabbit femoral artery thrombosis model - Enhancement of thrombolysis and improvement in capillary muscle perfusion
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DOI：
10.1161/01.cir.101.19.2296
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发表时间：
2000-05-16
期刊：
影响因子：
37.8
通讯作者：
Francis, CW
中科院分区：
文献类型：
--
作者：
Suchkova, VN;Baggs, RB;Francis, CW
背景-我们之前已经证明，低强度的40 khz超声（US）在体外加热少、组织渗透良好的情况下加速纤维蛋白溶解。这些研究现在已经扩展到在兔模型中检查40 khz US对溶栓和组织灌注的影响。方法和结果-治疗采用US单独0.75 W/cm(2)，单独使用链激酶，或US和链激酶联合使用。US或链激酶导致最小的溶栓，但120分钟后再灌注几乎完成。在没有动脉血流的情况下，US也逆转了非灌注肌肉的缺血。血栓形成后组织灌注从13.7+/-0.2 U下降到6.6+/-0.8 U， 240分钟后进一步下降到4.5+/-0.4 U。US在30分钟和60分钟后分别将灌注提高到10.6+/-0.5和12.1+/-0.5 U。这种效果是可逆的，停药后下降到预处理值。同样，血栓形成后，组织pH从正常下降到7.05+/-0.02，但US在60分钟后将pH提高到7.34+/-0.03。在股动脉结扎后，us诱导的组织灌注和pH值的改善也出现了，表明溶栓并没有引起这些影响。结论：低强度40khz US可显著加速纤维蛋白溶解，改善组织灌注，逆转酸中毒，有利于急性血栓的治疗。
Background - We have shown previously that 40-kHz ultrasound (US) at low intensity accelerates fibrinolysis in vitro with little heating and good tissue penetration. These studies have now been extended to examine the effects of 40-kHz US on thrombolysis and tissue perfusion in a rabbit model.Methods and Results - Treatment was administered with either US alone at 0.75 W/cm(2), streptokinase alone, or the combination of US and streptokinase. US or streptokinase resulted in minimal thrombolysis, but reperfusion was nearly complete with the combination after 120 minutes. US also reversed the ischemia in nonperfused muscle in the absence of arterial flow. Tissue perfusion decreased after thrombosis from 13.7+/-0.2 to 6.6+/-0.8 U and then declined further to 4.5+/-0.4 U after 240 minutes. US improved perfusion to 10.6+/-0.5 and 12.1+/-0.5 U after 30 and 60 minutes, respectively. This effect was reversible and declined to pretreatment values after US was discontinued. Similarly, tissue pH declined from normal to 7.05+/-0.02 after thrombosis, but US improved pH to 7.34+/-0.03 after 60 minutes. US-induced improvement in tissue perfusion and pH also occurred after femoral artery ligation, indicating that thrombolysis did not cause these effects.Conclusions - 40-kHz US at low intensity markedly accelerates fibrinolysis and also improves tissue perfusion and reverses acidosis, effects that would be beneficial in treatment of acute thrombosis.