Phage Lambda Capsids as Tunable Display Nanoparticles
Phage Lambda Capsids as Tunable Display Nanoparticles
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DOI：
10.1021/bm5011646
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发表时间：
2014-12-01
影响因子：
6.2
通讯作者：
Catalano, Carlos E.
中科院分区：
文献类型：
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作者：
Chang, Jenny R.;Song, Eun-Ho;Catalano, Carlos E.
纳米颗粒技术为开发用于治疗和诊断或治疗的生物医学应用的试剂提供了强大的工具。目前正在开发两大类粒子，病毒系统和合成系统，每种系统都有各自的优势和局限性。在这里，我们采用噬菌体Lambda系统来构建融合了这两种方法的模块化设计纳米颗粒。我们已经构建了各种修饰的装饰蛋白，允许用蛋白质和非蛋白质配体(包括小分子、碳水化合物和合成展示配体)对外壳进行位置特异性修饰。我们证明，嵌合蛋白可以用来同时以可调的表面密度装饰外壳，以提供物理上均一的颗粒，并且可以制造出以定义的组成显示各种配体的颗粒。这些设计的纳米颗粒为Lambda作为一种不可知的纳米颗粒系统的开发奠定了基础。
Nanoparticle technologies provide a powerful tool for the development of reagents for use in both therapeutic and diagnostic, or theragnostic biomedical applications. Two broad classes of particles are under development, viral and synthetic systems, each with their respective strengths and limitations. Here we adapt the phage lambda system to construct modular designer nanoparticles that blend these two approaches. We have constructed a variety of modified decoration proteins that allow site-specific modification of the shell with both protein and nonproteinaceous ligands including small molecules, carbohydrates, and synthetic display ligands. We show that the chimeric proteins can be used to simultaneously decorate the shell in a tunable surface density to afford particles that are physically homogeneous and that can be manufactured to display a variety of ligands in a defined composition. These designer nanoparticles set the stage for development of lambda as a theragnostic nanoparticle system.